Dkk-1 Proteins

3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (Catalog # 5439-DK) on days 5-7 to induce cardiomyocyte differentiation. Cells were then cultured in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond. Cells were fixed and stained for stage-specific markers at select time points during the procedure. The pluripotency marker Oct-4 was detected using a Mouse Anti-Human Oct-4A Monoclonal Antibody (MAB17591). The mesoderm marker, Snail is expressed intermediately during differentiation (Day 1). It was detected using a Goat Anti-Human Snail Polyclonal Antibody (AF3639). The cardiomyocyte markers NKX2.5 and Troponin T are not present in cells during early (Day 0) and intermediate (Day1) differentiation and become more highly expressed during the later stages of differentiation (Day 7, Day 30). NKX2.5 was detected using a Goat Anti-Human NKX2.5 Polyclonal Antibody (AF2444) and Troponin T was detected using a Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (MAB1874). Snail and NKX2.5 primary antibodies were visualized with a NorthernLights™ 557-conjugated Donkey Anti-Goat IgG Secondary Antibody (NL001). Oct-4 and Troponin T were visualized with a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (NL007)." class="big_lightbox"> 3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (Catalog # 5439-DK) on days 5-7 to induce cardiomyocyte differentiation. Cells were then cultured in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond. Cells were fixed and stained for stage-specific markers at select time points during the procedure. The pluripotency marker Oct-4 was detected using a Mouse Anti-Human Oct-4A Monoclonal Antibody (MAB17591). The mesoderm marker, Snail is expressed intermediately during differentiation (Day 1). It was detected using a Goat Anti-Human Snail Polyclonal Antibody (AF3639). The cardiomyocyte markers NKX2.5 and Troponin T are not present in cells during early (Day 0) and intermediate (Day1) differentiation and become more highly expressed during the later stages of differentiation (Day 7, Day 30). NKX2.5 was detected using a Goat Anti-Human NKX2.5 Polyclonal Antibody (AF2444) and Troponin T was detected using a Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (MAB1874). Snail and NKX2.5 primary antibodies were visualized with a NorthernLights™ 557-conjugated Donkey Anti-Goat IgG Secondary Antibody (NL001). Oct-4 and Troponin T were visualized with a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (NL007)." class="big_thumb" />

3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (Catalog # 5439-DK) on days 5-7. Following culture in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond, cells were fixed and stained for the atrial-specific marker, MLC2a, and the ventricle-specific marker, MYH7 using a Rabbit Anti-Human MYH7 Monoclonal Antibody (MAB90961)." class="big_lightbox"> 3434-010-02) and N21-MAX Insulin Free Media Supplement (AR010) along with Recombinant Human Activin A on day 0, Recombinant Human FGF-basic (4114-TC), Recombinant Human BMP-4, and CHIR99021 (4423) on days 1-5, and Recombinant Human Dkk-1 (Catalog # 5439-DK) on days 5-7. Following culture in media supplemented with N21-MAX Insulin Free Media Supplement (AR010) on days 7-12 and media supplemented with N21-MAX Media Supplement (AR008) from day 12 and beyond, cells were fixed and stained for the atrial-specific marker, MLC2a, and the ventricle-specific marker, MYH7 using a Rabbit Anti-Human MYH7 Monoclonal Antibody (MAB90961)." class="big_thumb" />

1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 1765-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 30-180 ng/mL (orange circles)." class="big_lightbox"> 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 1765-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 30-180 ng/mL (orange circles)." alt="Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Mouse Dkk-1 (Catalog # 1765-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 30-180 ng/mL (orange circles)." class="big_thumb" />

1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Rat Dkk-1 (Catalog # 4010-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 50-300 ng/mL (orange circles)." class="big_lightbox"> 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Rat Dkk-1 (Catalog # 4010-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 50-300 ng/mL (orange circles)." alt="Recombinant Mouse Wnt-3a (Catalog # 1324-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Rat Dkk-1 (Catalog # 4010-DK) inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 50-300 ng/mL (orange circles)." class="big_thumb" />

10450-DK) inhibits Recombinant Mouse Wnt-3a (1324-WN) induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 12.5-100 ng/mL." class="big_lightbox"> 10450-DK) inhibits Recombinant Mouse Wnt-3a (1324-WN) induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 12.5-100 ng/mL." alt="Recombinant Cynomolgus Monkey/Rhesus Macaque Dkk-1 (10450-DK) inhibits Recombinant Mouse Wnt-3a (1324-WN) induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 12.5-100 ng/mL." class="big_thumb" />

5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Biotinylated Human Dkk-1 (Catalog # BT5439) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a. The ED₅₀ for this effect is 10-60 ng/mL (orange circles)." class="big_lightbox"> 5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Biotinylated Human Dkk-1 (Catalog # BT5439) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a. The ED₅₀ for this effect is 10-60 ng/mL (orange circles)." alt="Recombinant Human Wnt-3a (Catalog # 5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Biotinylated Human Dkk-1 (Catalog # BT5439) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a. The ED₅₀ for this effect is 10-60 ng/mL (orange circles)." class="big_thumb" />

1324-WN) induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 8.00‑96.0 ng/mL." class="big_lightbox"> 1324-WN) induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 8.00‑96.0 ng/mL." alt="Recombinant Human Dkk-1 (Catalog # 11387-DK) inhibits Recombinant Mouse Wnt-3a (1324-WN) induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 8.00‑96.0 ng/mL." class="big_thumb" />

9734-CK) binds with an ED₅₀ of 15-90 ng/mL." class="big_lightbox"> 9734-CK) binds with an ED₅₀ of 15-90 ng/mL." alt="When Recombinant Mouse Dkk-1 N-Terminal Fragment (Catalog # 9839-DK) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse CKAP4/p63 (Catalog # 9734-CK) binds with an ED₅₀ of 15-90 ng/mL." class="big_thumb" />

9734-CK) binds with an ED₅₀ of 9-54 ng/mL." class="big_lightbox"> 9734-CK) binds with an ED₅₀ of 9-54 ng/mL." alt="When Recombinant Human Dkk-1 N-Terminal Fragment (Catalog # 9837-DK) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse CKAP4/p63 (Catalog # 9734-CK) binds with an ED₅₀ of 9-54 ng/mL." class="big_thumb" />