Recombinant Human DDB1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DDB1 Protein, CF Summary

• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1 protein
  Met1 - His1140
• Accession #: Q16531.1
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 127 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DDB1 Protein, CF

• damage-specific DNA binding protein 1 (127kD)
• damage-specific DNA binding protein 1, 127kDa
• Damage-specific DNA-binding protein 1
• DDB p127 Subunit
• DDB1
• DDBA
• DNA damage-binding protein 1
• DNA damage-binding protein a
• HBV X-associated protein 1
• UV-damaged DNA-binding factor
• UV-damaged DNA-binding protein 1
• UV-DDB 1
• UV-DDB1
• XAP1
• XAP-1
• Xeroderma pigmentosum group E-complementing protein
• XPCE
• XPE
• XPE-BF
• XPE-binding factor

Background

DNA damage-binding protein 1 (DDB1) is a component of the UV-damaged DNA binding (UV-DDB) complex which minimally consists of DDB1 and DDB2.  At 1,140 amino acids in length and a predicted molecular weight of 127 kDa, human DDB1 is 99% identical to both its mouse and rat orthologues.  DDB1 is also a component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin Ligase complexes where it serves to associate the Cullin-4 subunit to substrate recognition subunits such as Cereblon (CRBN), and various DCAF (DDB1-CUL4 Associate Factor) proteins.  

1. Fischer E.S. et al. (2014) Nature 512: 49.
2. He Y.J., et al. (2006) Genes Dev.  20: 2949.
3. Ito T., et al. (2010) Science 327: 1345.
4. Wang H., et al. (2006) Mol. Cell  22: 383.

Publications for DDB1 (E3-501)(1)

Publication using E3-501

• Chen, Q;Sun, P;Zhou, J;Long, T;Xiao, A;Liu, Z;Xu, S;Lei, W;Zhang, R;Tian, J;Zhou, M;Hu, Z;Zhu, F;Nie, J; Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice Nature communications 2025-07-24 [PMID: 40707439] (Bioassay)

Bioinformatics

• Uniprot:
  • Q16531.1()