Recombinant Human CXCL10/IP-10 Protein

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1 µg/lane of Recombinant Human CXCL10/IP-10/CRG-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.
Recombinant Human CXCL10/IP-10/CRG-2 (Catalog # 266-IP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3. The ED50 for this effect is 0.03-0.18 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CXCL10/IP-10 Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED50 for this effect is 0.03‑0.18 µg/mL.
Source
E. coli-derived human CXCL10/IP-10/CRG-2 protein
Val22-Pro98, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL10
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
266-IP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL10/IP-10 Protein

  • C7
  • chemokine (C-X-C motif) ligand 10
  • CRG2
  • CRG-2
  • CXCL10
  • gIP-10
  • IFI10
  • INP10
  • IP-10
  • mob-1
  • SCYB10

Background

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF‑ alpha, IL-12 and viruses. Additional cell types that have been shown to express CXCL10 include activated T‑lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, Crg-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T‑lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T‑lymphocytes.

  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al.(1996) J. Biol. Chem. 271:24286.

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Publications for CXCL10/IP-10/CRG-2 (266-IP)(40)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Virus.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA Standard, EnzAct.


Filter By Application
Binding Assay
(1)
Bioassay
(25)
ELISA Standard
(11)
EnzAct
(1)
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Human
(26)
Mouse
(1)
N/A
(11)
Virus
(1)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using 266-IP Applications Species
M Liu, W Solomon, JC Cespedes, NO Wilson, B Ford, JK Stiles Neuregulin-1 attenuates experimental cerebral malaria (ECM) pathogenesis by regulating ErbB4/AKT/STAT3 signaling J Neuroinflammation, 2018;15(1):104. 2018 [PMID: 29636063] (Bioassay, Human) Bioassay Human
Z Jiang, Y Li, X Ji, Y Tang, H Yu, L Ding, M Yu, Q Cui, M Zhang, Y Ma, M Li Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells Sci Rep, 2017;7(1):14510. 2017 [PMID: 29109449] (Bioassay, Human) Bioassay Human
B Schludi, ASM Moin, C Montemurro, T Gurlo, AV Matveyenko, D Kirakossia, DW Dawson, SM Dry, PC Butler, AE Butler Islet inflammation and ductal proliferation may be linked to increased pancreatitis risk in type 2 diabetes JCI Insight, 2017;2(13):. 2017 [PMID: 28679961] (Bioassay, Human) Bioassay Human
H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells Oncol Lett, 2017;13(6):4224-4230. 2017 [PMID: 28599423] (Bioassay, Human) Bioassay Human
N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence Nat Commun, 2017;8(0):15380. 2017 [PMID: 28541275] (Bioassay, Human) Bioassay Human
Jung Yong Hong Serum level of CXCL10 is associated with inflammatory prognostic biomarkers in patients with diffuse large B-cell lymphoma Hematol Oncol, 2016;0(0):. 2016 [PMID: 27943355] (Bioassay, Human) Bioassay Human
Valentina Cecchinato Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization J. Immunol, 2016;0(0):. 2016 [PMID: 27895171] (Bioassay, Human) Bioassay Human
Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies Mucosal Immunol, 2016;9(6):1584-1595. 2016 [PMID: 26883728] (Bioassay, Human) Bioassay Human
Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample. BMC Biotechnol, 2014;14(0):63. 2014 [PMID: 25022797] (ELISA Standard, N/A) ELISA Standard N/A
Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages. J Allergy Clin Immunol, 2014;133(4):1227-30. 2014 [PMID: 24529411] (Human) Human
Show All 40 Publications.

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Bioinformatics

Gene Symbol CXCL10
Entrez
Uniprot