Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human Chitinase 3-like 1/YKL-40 protein
  Tyr22-Thr383, with a C-terminal 6-His tag
• Accession #: P36222
• N-terminal Sequence: Tyr22
• Protein/Peptide Type: Recombinant Proteins
• Gene: CHI3L1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41.3 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38-41 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Chitinase 3-like 1/YKL-40 Protein, CF

• AW208766
• Brp39
• CHI3L1
• Chitinase 3 like 1
• Chitinase 3-like 1
• gp39
• HCgp39
• YKL-40

Background

Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family (1-4). CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells (1, 2, 5-7). The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides (3). Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins (1-4). CHI3L1 can bind heparins, probably as heparan sulfate (3, 7). It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis (4, 6-9). Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis (1, 9-12). Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood (13). CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary (9). In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis (9, 14, 15).

1. Hakala, B.E. et al. (1993) J. Biol. Chem. 268:25803.
2. Renkema, G.H. et al. (1998) Eur. J. Biochem. 251:504.
3. Fusetti, F. et al. (2003) J. Biol. Chem. 278:37753.
4. Houston, D.R. et al. (2003) J. Biol. Chem. 278:30206.
5. Volck, B. et al. (1998) Proc. Assoc. Am. Physicians 110:351.
6. Nishikawa, K.C. and A.J.T. Millis (2003) Exp. Cell Res. 287:79.
7. Shao, R. et al. (2009) Oncogene 28:4456.
8. Recklies, A.D. et al. (2002) Biochem. J. 365:119.
9. Eurich, K. et al. (2009) World J. Gastroenterol. 15:5249.
10. Letuve, S. et al. (2008) J Immunol. 181:5167.
11. Rathke, C.N. and H. Vestergaard (2009) Cardiovasc. Diabetol. 8:61.
12. Johansen, J.S. et al. (1997) Scand. J. Gastroenterol. 32:582.
13. Ober, C. et al. (2008) N. Engl. J. Med. 358:1682.
14. Johansen, J.S. et al. (1999) Rheumatology 38:618.
15. Chupp, G.L. et al. (2007) N. Engl. J. Med. 357:2016.

Publications for Chitinase 3-like 1/YKL-40 (2599-CH)(6)

Publications using 2599-CH

• Wang, YG;Lin, C;Huang, M;Fang, XL;Chen, GH;Ye, SN; Overexpression of YKL40,IL-6, IL-8, TNF-? in tonsils and the role of YKL40 in childhood with obstructive sleep apnea syndrome Scientific reports 2024-11-01 [PMID: 39487152] (Bioassay, Human)
• Yamanaka, K;Koma, YI;Urakami, S;Takahashi, R;Nagamata, S;Omori, M;Torigoe, R;Yokoo, H;Nakanishi, T;Ishihara, N;Tsukamoto, S;Kodama, T;Nishio, M;Shigeoka, M;Yokozaki, H;Terai, Y; YKL40/Integrin ?4 Axis Induced by the Interaction between Cancer Cells and Tumor-Associated Macrophages Is Involved in the Progression of High-Grade Serous Ovarian Carcinoma International journal of molecular sciences 2024-10-01 [PMID: 39408927] (Bioassay, Human)
• Song, Y;Jiang, W;Afridi, SK;Wang, T;Zhu, F;Xu, H;Nazir, FH;Liu, C;Wang, Y;Long, Y;Huang, YA;Qiu, W;Tang, C; Astrocyte-derived CHI3L1 signaling impairs neurogenesis and cognition in the demyelinated hippocampus Cell reports 2024-05-10 [PMID: 38733586] (Bioassay, Mouse)
• Jiang, W;Zhu, F;Xu, H;Xu, L;Li, H;Yang, X;Khan Afridi, S;Lai, S;Qiu, X;Liu, C;Li, H;Long, Y;Wang, Y;Connolly, K;Elias, JA;Lee, CG;Cui, Y;Huang, YA;Qiu, W;Tang, C; CHI3L1 signaling impairs hippocampal neurogenesis and cognitive function in autoimmune-mediated neuroinflammation Science advances 2023-09-29 [PMID: 37756391] (Cell Culture, Mouse)
• SC Starossom, J Campo Garc, T Woelfle, S Romero-Sua, M Olah, F Watanabe, L Cao, A Yeste, JJ Tukker, FJ Quintana, J Imitola, F Witzel, D Schmitz, M Morkel, F Paul, C Infante-Du, SJ Khoury Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation Nat Commun, 2019-01-15;10(1):217. 2019-01-15 [PMID: 30644388] (Bioassay, Human)
• Park JA, Drazen JM, Tschumperlin DJ The chitinase-like protein YKL-40 is secreted by airway epithelial cells at base line and in response to compressive mechanical stress. J. Biol. Chem., 2010-07-22;285(39):29817-25. 2010-07-22 [PMID: 20650887] (Western Blot)

Bioinformatics

• Gene Symbol: CHI3L1
• Uniprot:
  • P36222()