Recombinant Human CCL4/MIP-1 beta Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CCL4/MIP-1 beta Protein, CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 1‑6 ng/mL.
Source
E. coli-derived human CCL4/MIP-1 beta protein
Ala24-Asn92 (Major); Pro30-Asn92 (minor)
Accession #
N-terminal Sequence
Ala24 (major); Pro30 (minor)
Protein/Peptide Type
Recombinant Proteins
Gene
CCL4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.8 kDa (major) & 7.3 kDa (minor).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
271-BME/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL4/MIP-1 beta Protein, CF

  • ACT2
  • ACT-2
  • AT744.1
  • C-C motif chemokine 4
  • CCL4
  • chemokine (C-C motif) ligand 4
  • Exodus-3
  • G-26 T-lymphocyte-secreted protein
  • G-26
  • HC21
  • LAG1
  • LAG-1
  • Lymphocyte activation gene 1 protein
  • Macrophage inflammatory protein 1-beta
  • MIP1 beta
  • MIP-1 beta
  • MIP1B
  • MIP1B1
  • MIP-1-beta
  • MIP-1-beta(1-69)
  • PAT 744
  • Protein H400
  • SCYA2
  • SCYA4
  • secreted protein G-26
  • SIS-gamma
  • small inducible cytokine A4 (homologous to mouse Mip-1b)
  • Small-inducible cytokine A4
  • T-cell activation protein 2

Background

CCL4, also known as macrophage inflammatory protein 1 beta (MIP-1 beta ) is a 7.8 kDa beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells (1, 2). CCL4 attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium (3 - 8). A CCL4 deficiency in mice promotes the development of autoantibodies, possibly as a result of compromised regulatory T cell recruitment (6). CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha (9). The first two N-terminal amino acids can be cleaved from human CCL4 by CD26/DPPIV (10, 11). Both the full length and truncated forms exert biological activity through CCR5, and the truncated form additionally interacts with CCR1 and CCR2b (10). In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor (2). Both forms of CCL4 block HIV-1 infection of T cells by inducing the downregulation of CCR5 (10). Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively. 
  1. Rot, A. and U.H. von Andrian (2004) Annu. Rev. Immunol. 22:891.
  2. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  3. Sun, X. et al. (2006) Infec. Immun. 74:5943.
  4. Bisset, L.R. and Schmid-Grendelmeier, P. (2005) Curr. Opin. Pulm. Med. 11:35.
  5. Frangogiannis, N.G. (2004) Inflamm. Res. 53:585.
  6. Bystry, R.S. et al. (2001) Nat. Immunol. 2:1126.
  7. Oliveira, S.H.P. et al. (2002) J. Leukoc. Biol. 71:1019.
  8. Schall, T.J. et al. (1993) J. Exp. Med. 177:1821.
  9. Guan, E. et al. (2001) J. Biol. Chem. 276:12404.
  10. Guan, E. et al. (2002) J. Biol. Chem. 277:32348.
  11. Guan, E. et al. (2004) J. Cell. Biochem. 92:53.

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Publications for CCL4/MIP-1 beta (271-BME/CF)(21)

We have publications tested in 4 confirmed species: Human, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA Standard, Hemofiltration Procedure.


Filter By Application
Binding Assay
(1)
Bioassay
(15)
ELISA Standard
(3)
Hemofiltration Procedure
(1)
All Applications
Filter By Species
Human
(14)
N/A
(3)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Virus
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using 271-BME/CF Applications Species
Benjamin Malard Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study Artif Organs, 2016;0(0):. 2016 [PMID: 27911005] (Hemofiltration Procedure, Human) Hemofiltration Procedure Human
Del Corno M, Donninelli G, Varano B, Da Sacco L, Masotti A, Gessani S HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells. J Virol, 2014;88(19):11045-55. 2014 [PMID: 25008924] (Bioassay, Human) Bioassay Human
Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J CCR5 is a receptor for Staphylococcus aureus leukotoxin ED. Nature, 2013;493(7430):51-5. 2013 [PMID: 23235831] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Badr G, Lefevre EA, Mohany M Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE, 2011;6(9):e23741. 2011 [PMID: 21912642] (Bioassay, Human) Bioassay Human
Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B. Immunology, 2010;129(4):536-46. 2010 [PMID: 20002784] (Bioassay, Human) Bioassay Human
Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008;181(11):8053-67. 2008 [PMID: 19017998] (Bioassay, Human) Bioassay Human
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J. Leukoc. Biol., 2008;84(4):1109-19. 2008 [PMID: 18591415] (Bioassay, Human) Bioassay Human
Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation. Blood, 2008;112(3):493-503. 2008 [PMID: 18480427] (Bioassay, Human) Bioassay Human
Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes. Blood, 2008;111(7):3579-90. 2008 [PMID: 18203954] (Bioassay, Human) Bioassay Human
Show All 21 Publications.

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Bioinformatics

Gene Symbol CCL4
Uniprot