Recombinant Human CCL25/TECK Protein

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Recombinant Human CCL25/TECK (Catalog #9046-TK) chemoattracts the BaF3 pro-B cells transfected with human CCR9. The ED50 for this effect is 30-180 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human CCL25/TECK Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR9. The ED50 for this effect is 30-180 ng/mL.
Source
E. coli-derived human CCL25/TECK protein
Gln24-Leu150
Accession #
N-terminal Sequence
Gln24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL25
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
Publications
Read Publication using
9046-TK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL25/TECK Protein

  • C-C motif chemokine 25
  • CCL25
  • chemokine (C-C motif) ligand 25
  • Chemokine TECK
  • Ck beta-15
  • Ckb15
  • SCYA25TECKvar
  • small inducible cytokine subfamily A (Cys-Cys), member 25
  • Small-inducible cytokine A25
  • TECK
  • TECKMGC150327
  • thymus expressed chemokine
  • Thymus-expressed chemokine

Background

CCL25, also known as TECK (thymus-expressed chemokine), is a CC chemokine that regulates the trafficking of lymphocytes in the thymus and small intestine. Mature human CCL25 shares 40% amino acid sequence identity with mouse and rat CCL25 (1). CCL25 is produced by stromal cells in the thymus and epithelial cells of the small intestine, particularly the jejunum and ileum (1-3). It binds to and induces chemoattraction through CCR9 (1, 4, 5), and both human and mouse proteins act on human CCR9 (4). CCR9 is expressed on immature pre-T cells and thymocytes (5, 6). CCL25 induces the homing of several lymphocyte populations to the small intestine (3), including Integrin alpha 4 beta 7+ gamma δ T cells (6, 7), Integrin alpha E beta 7+ CD8+ T cells (8), and IgA-producing plasma cells (2). In cancer, functional CCR9 mediates the metastasis of melanoma cells to the small intestine (9), contributes to the CCL25-dependent migration and invasion of some breast carcinomas (10), and attracts mesenchymal stromal cells to CCL25-expressing multiple myelomas (11). CCL25 contributes to the severity of chronic inflammation in rheumatoid arthritis where it attracts CCR9+ monocytes and macrophages (12), in endometriosis where it promotes the invasiveness of stromal cells (13), and in atherosclerosis where it contributes to the accumulation of CCR9+ macrophages in arterial plaques (14).
  1. Vicari, A.P. et al. (1997) Immunity 7:291.
  2. Bowman, E.P. et al. (2002) J. Exp. Med. 195:269.
  3. Kunkel, E.J. et al. (2000) J. Exp. Med. 192:761.
  4. Zaballos, A. et al. (1999) J. Immunol. 162:5671.
  5. Youn, B.-S. et al. (1999) Blood 94:2533.
  6. Uehara, S. et al. (2002) J. Immunol. 168:134.
  7. Costa, M.F.S. et al. (2012) Eur. J. Immunol. 42:1250.
  8. Svensson, M. et al. (2002) J. Clin. Invest. 110:1113.
  9. Amersi, F.F. et al. (2008) Clin. Cancer Res. 14:638.
  10. Johnson-Holiday, C. et al. (2011) Int. J. Oncol. 38:1279.
  11. Xu, S. et al. (2012) Stem Cells 30:266.
  12. Schmutz, C. et al. (2010) Arthritis Res. Ther. 12:R161.
  13. Wang, Y. et al. (2010) Cell. Mol. Immunol. 7:51.
  14. Alla, J.A. et al. (2010) J. Biol. Chem. 285:23496.

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Publications for CCL25/TECK (9046-TK)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol CCL25
Uniprot