Recombinant Human CCL20/MIP-3 alpha Protein, CF

Recombinant Human CCL20/MIP-3 alpha (Catalog # 360-MP/CF) chemo­attracts the BaF3 mouse pro-B cells transfected with human CCR6. The ED50 for this effect is 0.5‑2 ng/mL.

1 μg/lane of Recombinant Human CCL20/MIP-3 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CCL20/MIP-3 alpha Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR6. The ED₅₀ for this effect is 0.5‑2 ng/mL.
• Source: E. coli-derived human CCL20/MIP-3 alpha protein
  Ala27-Met96 (Asn95Asp)
• Accession #: P78556
• N-terminal Sequence: Ala27
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL20
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL20/MIP-3 alpha Protein, CF

• beta chemokine exodus-1
• Beta-chemokine exodus-1
• CC chemokine LARC
• C-C motif chemokine 20
• CCL20
• chemokine (C-C motif) ligand 20
• CKb4
• exodus-1
• LARC
• LARCLiver and activation-regulated chemokine
• MIP3 alpha
• MIP-3 alpha
• MIP-3a
• MIP-3-alpha
• MIP3AMacrophage inflammatory protein 3 alpha
• SCYA20Small-inducible cytokine A20
• small inducible cytokine subfamily A (Cys-Cys), member 20
• ST38

Background

CCL20, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. CCL20 cDNA encodes a 96 amino acid (aa) residue precursor protein with a 26 aa residue signal peptide that is predicted to be cleaved to form the 70 aa residue mature secreted protein. CCL20 is distantly related to other beta chemokines (20 - 28% aa sequence identity) and the gene for CCL20 has been mapped to chromosome 2 rather than 17.

CCL20 has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. The expression of CCL20 is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant CCL20 has been shown to be chemotactic for lymphocytes and to inhibit proliferation of myeloid progenitors in colony formation assays. CCL20 has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34⁺ cord blood precursors.

1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
2. Hromas, R. et al. (1997) Blood 89:3315.
3. Greaves, D.R. et al. (1997) J. Exp. Med. 186:837.

Publications for CCL20/MIP-3 alpha (360-MP/CF)(22)

Publications using 360-MP/CF

• P Esteves, B Allard, A Celle, I Dupin, E Maurat, O Ousova, M Thumerel, JW Dupuy, T Leste-Lass, R Marthan, PO Girodet, T Trian, P Berger Asthmatic bronchial smooth muscle increases rhinovirus replication within the bronchial epithelium Cell Reports, 2022-03-29;38(13):110571. 2022-03-29 [PMID: 35354045] (Bioassay, Human)
• KC Chang, RY Chen, YC Wang, LY Hung, LJ Medeiros, YP Chen, TY Chen, JC Yang, PM Chiang Stem cell characteristics promote aggressiveness of diffuse large B-cell lymphoma Scientific Reports, 2020-12-07;10(1):21342. 2020-12-07 [PMID: 33288848] (Bioassay, Human)
• M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals Mucosal Immunol, 2020-04-28;0(0):. 2020-04-28 [PMID: 32346082] (Bioassay, Human)
• HK Skovdahl, JK Damås, AVB Granlund, AE Østvik, B Doseth, T Bruland, TE Mollnes, AK Sandvik C-C Motif Ligand 20 (CCL20) and C-C Motif Chemokine Receptor 6 (CCR6) in Human Peripheral Blood Mononuclear Cells: Dysregulated in Ulcerative Colitis and a Potential Role for CCL20 in IL-1? Release Int J Mol Sci, 2018-10-20;19(10):. 2018-10-20 [PMID: 30347808] (Bioassay, Human)
• R Diao, T Wang, KL Fok, X Li, Y Ruan, MK Yu, Y Cheng, Y Chen, H Chen, L Mou, X Cai, Y Wang, Z Cai, X Zeng, HC Chan CCR6 is required for ligand-induced CatSper activation in human sperm Oncotarget, 2017-09-05;8(53):91445-91458. 2017-09-05 [PMID: 29207656] (Bioassay, Human)
• G Lu, X Zhang, L Shen, Q Qiao, Y Li, J Sun, J Zhang CCL20 secreted from IgA1-stimulated human mesangial cells recruits inflammatory Th17 cells in IgA nephropathy PLoS ONE, 2017-05-26;12(5):e0178352. 2017-05-26 [PMID: 28552941] (Bioassay, Human)
• Valentina Cecchinato Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization J. Immunol, 2016-11-28;0(0):. 2016-11-28 [PMID: 27895171] (Bioassay, Human, Primate - Macaca mulatta (Rhesus Macaque))
• Andy T Y Lau Aberrant cytokine secretion and zinc uptake in chronic cadmium-exposed lung epithelial cells Proteomics Clin Appl, 2016-12-14;0(0):. 2016-12-14 [PMID: 27801555] (ELISA (Standard))
• Yu , Qing, Lou , Xiang-mi, He , Yan Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway. PLoS ONE, 2015-03-13;10(3):e0120855. 2015-03-13 [PMID: 25768730] (Bioassay, Human)
• Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells. Invest Ophthalmol Vis Sci, 2015-01-20;56(2):1051-62. 2015-01-20 [PMID: 25604685] (Bioassay, Human)
• Du , Dongshu, Liu , Yeliu, Qian , Haixin, Zhang , Bo, Tang , Xiaojun, Zhang , Ti, Liu , Weidong The effects of the CCR6/CCL20 biological axis on the invasion and metastasis of hepatocellular carcinoma. Int J Mol Sci, 2014-04-16;15(4):6441-52. 2014-04-16 [PMID: 24743888] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Kim S, Lewis C, Nadel JA CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells. J. Immunol., 2011-02-07;186(6):3392-400. 2011-02-07 [PMID: 21300824] (Bioassay, Human)
• Shan M, Cheng HF, Song LZ Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema. Sci Transl Med, 2009-10-28;1(4):4ra10. 2009-10-28 [PMID: 20368170] (Bioassay, Human)
• Charles J, Di Domizio J, Salameire D, Bendriss-Vermare N, Aspord C, Muhammad R, Lefebvre C, Plumas J, Leccia MT, Chaperot L Characterization of circulating dendritic cells in melanoma: role of CCR6 in plasmacytoid dendritic cell recruitment to the tumor. J. Invest. Dermatol., 2010-03-11;130(6):1646-56. 2010-03-11 [PMID: 20220766] (Bioassay, Human)
• Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling. J. Exp. Med., 2009-03-09;206(3):535-48. 2009-03-09 [PMID: 19273625] (Bioassay, Human)
• Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis. J. Leukoc. Biol., 2007-07-05;82(4):946-55. 2007-07-05 [PMID: 17615381] (Bioassay, Human)
• Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood, 2006-12-29;109(9):3649-57. 2006-12-29 [PMID: 17197436] (Bioassay, Human)
• Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium. J. Exp. Med., 2006-11-20;203(12):2763-77. 2006-11-20 [PMID: 17116732] (Bioassay, Human)
• Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. J. Immunol., 2006-10-01;177(7):4488-94. 2006-10-01 [PMID: 16982885] (Bioassay, Human)
• Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006-04-17;350(2):484-92. 2006-04-17 [PMID: 16603217] (Binding Assay, Bioassay, Virus)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CCL20
• Uniprot:
  • P78556()