Recombinant Human CCL18/PARC Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CCL18/PARC Protein Summary

Details of Functionality
Measured by its ability to inhibit Eotaxin-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR3. Nibbs, RJB. et al. (2000) J. Immunol. 164:1488. The ED50 for this effect is 1-5 µg/mL.
Source
E. coli-derived human CCL18/PARC protein
Ala21-Ala89
Accession #
N-terminal Sequence
Ala21
Protein/Peptide Type
Recombinant Proteins
Gene
CCL18
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
394-PA in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL18/PARC Protein

  • Alternative macrophage activation-associated CC chemokine 1
  • AMAC-1
  • AMAC1CC chemokine ligand 18
  • AMAC-1Small-inducible cytokine A18
  • CCL18
  • chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
  • CKb7
  • DC-CK1
  • DC-CK1CC chemokine PARC
  • DCCK1Macrophage inflammatory protein 4
  • Dendritic cell chemokine 1
  • MIP4
  • MIP-4
  • MIP-4C-C motif chemokine 18
  • PARC
  • PARCPulmonary and activation-regulated chemokine
  • SCYA18chemokine (C-C), dendritic
  • small inducible cytokine A18
  • small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Background

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM‑CSF/IL‑4‑induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.

  1. Adema, G. et al. (1997) Nature 387:713.
  2. Kodelja, V. et al. (1998) J. Immunol. 160:141.

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Publications for CCL18/PARC (394-PA)(8)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Virus.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA (Standard), In Vivo.


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Binding Assay
(1)
Bioassay
(4)
ELISA (Standard)
(2)
In Vivo
(1)
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Human
(4)
Mouse
(1)
N/A
(2)
Virus
(1)
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Showing Publications 1 - 8 of 8.
Publications using 394-PA Applications Species
Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28533380] (Bioassay, Human) Bioassay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Deegan PB, Pavlova E, Tindall J Osseous manifestations of adult Gaucher disease in the era of enzyme replacement therapy. Medicine (Baltimore), 2011;90(1):52-60. 2011 [PMID: 21200186] (ELISA (Standard), N/A) ELISA (Standard) N/A
Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM Potential biomarkers of osteonecrosis in Gaucher disease. Blood Cells Mol. Dis., 2011;46(1):27-33. 2011 [PMID: 21075651] (ELISA (Standard), N/A) ELISA (Standard) N/A
Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B. Immunology, 2010;129(4):536-46. 2010 [PMID: 20002784] (Bioassay, Human) Bioassay Human
Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006;350(2):484-92. 2006 [PMID: 16603217] (Binding Assay, Virus) Binding Assay Virus
Gunther C, Bello-Fernandez C, Kopp T, Kund J, Carballido-Perrig N, Hinteregger S, Fassl S, Schwarzler C, Lametschwandtner G, Stingl G, Biedermann T, Carballido JM CCL18 is expressed in atopic dermatitis and mediates skin homing of human memory T cells. J. Immunol., 2005;174(3):1723-8. 2005 [PMID: 15661937] (In Vivo, Mouse) In Vivo Mouse
Wan Y, Jakway JP, Qiu H, Shah H, Garlisi CG, Tian F, Ting P, Hesk D, Egan RW, Billah MM, Umland SP Identification of full, partial and inverse CC chemokine receptor 3 agonists using [35S]GTPgammaS binding. Eur. J. Pharmacol., 2002;456(1):1-10. 2002 [PMID: 12450563] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol CCL18
Uniprot