Recombinant Human CCL11/Eotaxin Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CCL11/Eotaxin Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 1-5 ng/mL.
Source
E. coli-derived human CCL11/Eotaxin protein
Gly24-Pro97
Accession #
N-terminal Sequence
Gly24
Protein/Peptide Type
Recombinant Proteins
Gene
CCL11
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
320-EO in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL11/Eotaxin Protein

  • C-C motif chemokine 11
  • CCL11
  • chemokine (C-C motif) ligand 11
  • Eosinophil chemotactic protein
  • Eotaxin
  • eotaxin-1
  • MGC22554
  • SCYA11Small-inducible cytokine A11
  • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Background

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Microsequencing of the purified protein revealed the guinea pig CCL11 to be a member of the beta (CC) chemokine family of inflammatory and immunoregulatory cytokines. cDNA clones for guinea pig, mouse and human CCL11 have been isolated. Human CCL11 cDNA encodes a 97 amino acid residue precursor protein from which the amino-terminal 23 amino acid residues are cleaved to generate the 74 amino acid residue mature human CCL11. At the protein sequence level, mature human CCL11 is approximately 60% identical to mature mouse and guinea pig CCL11. In addition, human CCL11 also shows high amino acid sequence identity to human MCP-1, 2 and 3. Human CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. The CC chemokine receptor 3 (CCR3) has now been identified to be a specific human CCL11 receptor (1 ‑ 3). CCR3 has also been shown to serve as a cofactor for a restricted subset of primary HIV viruses and binding of CCL11 to CCR3 inhibited infection by the HIV isolates (4).

  1. Kitamura, M. et al. (1996) J. Biol. Chem 271:7725.
  2. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
  3. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  4. Choe, H. et al. (1996) Cell 85:1135.
 

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Publications for CCL11/Eotaxin (320-EO)(26)

We have publications tested in 3 confirmed species: Human, N/A, Virus.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA (Standard), Hemofiltration Procedure.


Filter By Application
Binding Assay
(1)
Bioassay
(20)
ELISA (Standard)
(3)
Hemofiltration Procedure
(1)
All Applications
Filter By Species
Human
(21)
N/A
(3)
Virus
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using 320-EO Applications Species
K de Ruiter, S van Staver, B Hilvering, E Knol, N Vrisekoop, L Koenderman, M Yazdanbakh A field-applicable method for flow cytometric analysis of granulocyte activation: Cryopreservation of fixed granulocytes Cytometry A, 2018;0(0):. 2018 [PMID: 29533506] (Bioassay, Human) Bioassay Human
Benjamin Malard Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study Artif Organs, 2016;0(0):. 2016 [PMID: 27911005] (Hemofiltration Procedure, Human) Hemofiltration Procedure Human
Rossana C N Melo Extracellular Microvesicle Production by Human Eosinophils Activated by Inflammatory Stimuli Front Cell Dev Biol, 2016;4(0):117. 2016 [PMID: 27833910] (Bioassay, Human) Bioassay Human
Edson Antunes The soluble guanylyl cyclase activator BAY 60-2770 inhibits murine allergic airways inflammation and human eosinophil chemotaxis Pulm Pharmacol Ther, 2016;0(0):. 2016 [PMID: 27816773] (Bioassay, Human) Bioassay Human
Anti-Inflammatory Effects of Metformin Irrespective of Diabetes Status Circ Res, 2016;0(0):. 2016 [PMID: 27418629] (Bioassay, Human) Bioassay Human
Tahapary D, de Ruiter K, Martin I, van Lieshout L, Guigas B, Soewondo P, Djuardi Y, Wiria A, Mayboroda O, Houwing-Duistermaat J, Tasman H, Sartono E, Yazdanbakhsh M, Smit J, Supali T Helminth infections and type 2 diabetes: a cluster-randomized placebo controlled SUGARSPIN trial in Nangapanda, Flores, Indonesia. BMC Infect Dis, 2015;15(0):133. 2015 [PMID: 25888525] (Bioassay, Human) Bioassay Human
Konno, Yasunori, Ueki, Shigehar, Takeda, Masahide, Kobayashi, Yoshiki, Tamaki, Mami, Moritoki, Yuki, Oyamada, Hajime, Itoga, Masamich, Kayaba, Hiroyuki, Omokawa, Ayumi, Hirokawa, Makoto Functional analysis of free fatty acid receptor GPR120 in human eosinophils: implications in metabolic homeostasis. PLoS ONE, 2015;10(3):e0120386. 2015 [PMID: 25790291] (Bioassay, Human) Bioassay Human
Morshed M, Hlushchuk R, Simon D, Walls A, Obata-Ninomiya K, Karasuyama H, Djonov V, Eggel A, Kaufmann T, Simon H, Yousefi S NADPH oxidase-independent formation of extracellular DNA traps by basophils. J Immunol, 2014;192(11):5314-23. 2014 [PMID: 24771850] (Bioassay, Human) Bioassay Human
Zhu F, Liu P, Li J, Zhang Y Eotaxin-1 promotes prostate cancer cell invasion via activation of the CCR3-ERK pathway and upregulation of MMP-3 expression. Oncol Rep, 2014;31(5):2049-54. 2014 [PMID: 24604010] (Bioassay, Human) Bioassay Human
Melo, Rossana, Paganoti, Guillher, Dvorak, Ann M, Weller, Peter F The internal architecture of leukocyte lipid body organelles captured by three-dimensional electron microscopy tomography. PLoS ONE, 2013;8(3):e59578. 2013 [PMID: 23555714] (Bioassay, Human) Bioassay Human
Show All 26 Publications.

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Bioinformatics

Gene Symbol CCL11
Uniprot