Recombinant Human BMP-2/BMP-6 Heterodimer Protein

Images

 
There are currently no images for BMP-2/BMP-6 Heterodimer (7145-BP).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human BMP-2/BMP-6 Heterodimer Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.

The ED50 for this effect is 4-20 ng/mL.

Source
E. coli-derived human BMP-2/BMP-6 Heterodimer protein
Human BMP-2
(Ala284 - Arg396), with an N-terminal Met
Accession # P12643
Human BMP-6
(Gln382 - His513), with an N-terminal Met
Accession # P22004
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala284 (BMP-2) & Met (BMP-6)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.8 kDa (BMP-2) & 15 kDa (BMP-6).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
11 kDa & 14 kDa, reducing conditions
Publications
Read Publications using
7145-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-2/BMP-6 Heterodimer Protein

  • BMP-2/BMP-6 Heterodimer

Background

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, and BMP-2 are members of the BMP family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (1 - 4). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (5). Human BMP-6 is synthesized as a 513 aa precursor protein that contains a 20 aa signal sequence, a 354 aa prosegment, and a 139 aa mature region (6). BMP prosegments are removed by proteolysis, enabling the glycosylated 18 kDa mature BMPs to form active disulfide-linked homodimers or heterodimers (1, 2). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2, and mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. They share 48% aa sequence identity with each other. Both BMP-2 and BMP-6 induce osteogenic and chondrogenic differentiation in mesenchymal stem cells (4). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (7, 8). BMP-2/6 heterodimers also show increased activity at inducing trophoectodermal and endodermal differentiation of embryonic stem cells compared to either homodimer (9).
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Bragdon, B. et al. (2010) Cell Signal. 23:609.
  3. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
  4. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
  5. Wozney, J. et al. (1988) Science 242:1528.
  6. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
  7. Isaacs, M.J. et al. (2010) Mol. Endocrinol. 24:1469.
  8. Israel, D.I. et al. (1996) Growth Factors 13:291.
  9. Valera, E. et al. (2010) PLoS ONE 5:e11167.

Customers Who Viewed This Item Also Viewed...

355-BM/CF
Species: Hu, Mu, Rt
Applications: BA
314-BP
Species: Hu
Applications: BA, BA
MAB1419
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
354-BP
Species: Hu
Applications: BA
NBP1-77461
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
DPI00
Species: Hu
Applications: ELISA
NB110-3638
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI, WB
NBP1-89131
Species: Hu
Applications: ICC/IF, IHC, IHC-P
DCC270
Species: Hu
Applications: ELISA
NBP1-89133
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89134
Species: Hu
Applications: IHC, IHC-P
NBP2-32263
Species: Hu, Mu
Applications: IHC, IP, WB
NB100-56443
Species: Hu, Mu, Rt
Applications: WB
NBP1-85533
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF808
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, IHC, Neut, WB
507-BP
Species: Hu
Applications: BA
6057-NG
Species: Hu
Applications: BA
233-FB/CF
Species: Hu
Applications: BA
DVE00
Species: Hu
Applications: ELISA
464-SH/CF
Species: Mu
Applications: BA

Publications for BMP-2/BMP-6 Heterodimer (7145-BP)(3)

Reviews for BMP-2/BMP-6 Heterodimer (7145-BP) (0)

There are no reviews for BMP-2/BMP-6 Heterodimer (7145-BP). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for BMP-2/BMP-6 Heterodimer (7145-BP) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional BMP-2/BMP-6 Heterodimer Products

Bioinformatics Tool for BMP-2/BMP-6 Heterodimer (7145-BP)

Discover related pathways, diseases and genes to BMP-2/BMP-6 Heterodimer (7145-BP). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BMP-2/BMP-6 Heterodimer (7145-BP)

Discover more about diseases related to BMP-2/BMP-6 Heterodimer (7145-BP).
 

Pathways for BMP-2/BMP-6 Heterodimer (7145-BP)

View related products by pathway.

PTMs for BMP-2/BMP-6 Heterodimer (7145-BP)

Learn more about PTMs related to BMP-2/BMP-6 Heterodimer (7145-BP).

Blogs on BMP-2/BMP-6 Heterodimer

There are no specific blogs for BMP-2/BMP-6 Heterodimer, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human BMP-2/BMP-6 Heterodimer Protein and receive a gift card or discount.

Bioinformatics