Recombinant Human BMP-2/BMP-6 Heterodimer Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human BMP-2/BMP-6 Heterodimer Protein, CF Summary

• Details of Functionality: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272.

  The ED₅₀ for this effect is 4-20 ng/mL.

• Source: E. coli-derived human BMP-2/BMP-6 Heterodimer protein
  

  Human BMP-2 (Ala284 - Arg396), with an N-terminal Met Accession # P12643

  Human BMP-6 (Gln382 - His513), with an N-terminal Met Accession # P22004
  N-terminus	C-terminus

• Accession #: P12643 (BMP-2) & P22004 (BMP-6)
• N-terminal Sequence: Ala284 (BMP-2) & Met (BMP-6)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: BMP2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12.8 kDa (BMP-2) & 15 kDa (BMP-6).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 11 kDa & 14 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-2/BMP-6 Heterodimer Protein, CF

• BMP-2/BMP-6 Heterodimer

Background

Bone Morphogenetic Protein 6 (BMP-6), also known as Vgr-1, and BMP-2 are members of the BMP family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs are involved in a wide range of processes including embryogenesis, tissue morphogenesis, cell differentiation and migration, and tumorigenesis. Cellular responses to BMPs are mediated by hetero‑oligomeric complexes of type I and type II serine/threonine kinase receptors (1 - 4). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (5). Human BMP-6 is synthesized as a 513 aa precursor protein that contains a 20 aa signal sequence, a 354 aa prosegment, and a 139 aa mature region (6). BMP prosegments are removed by proteolysis, enabling the glycosylated 18 kDa mature BMPs to form active disulfide-linked homodimers or heterodimers (1, 2). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2, and mature human BMP-6 shares 96% and 98% aa sequence identity with mouse and rat BMP-6, respectively. They share 48% aa sequence identity with each other. Both BMP-2 and BMP-6 induce osteogenic and chondrogenic differentiation in mesenchymal stem cells (4). Heterodimers of BMP-2 and BMP-6 show increased potency at inducing osteoblastic calcium deposition, chondrogenesis, and in vivo bone formation compared to either BMP-2 or BMP-6 homodimers (7, 8). BMP-2/6 heterodimers also show increased activity at inducing trophoectodermal and endodermal differentiation of embryonic stem cells compared to either homodimer (9).

1. Chen, D. et al. (2004) Growth Factors 22:233.
2. Bragdon, B. et al. (2010) Cell Signal. 23:609.
3. Singh, A. and R.J. Morris (2010) Cytokine Growth Factor Rev. 21:299.
4. Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
5. Wozney, J. et al. (1988) Science 242:1528.
6. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
7. Isaacs, M.J. et al. (2010) Mol. Endocrinol. 24:1469.
8. Israel, D.I. et al. (1996) Growth Factors 13:291.
9. Valera, E. et al. (2010) PLoS ONE 5:e11167.

Publications for BMP-2/BMP-6 Heterodimer (7145-BP/CF)(3)

Publications using 7145-BP/CF

• A Krouwels, JD Iljas, AHM Kragten, WJA Dhert, FC Öner, MA Tryfonidou, LB Creemers Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration Int J Mol Sci, 2020-04-14;21(8):. 2020-04-14 [PMID: 32295299] (Bioassay, Human)
• E Toyoda, M Sato, T Takahashi, M Maehara, Y Nakamura, G Mitani, T Takagaki, K Hamahashi, M Watanabe Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue Regen Ther, 2018-11-20;10(0):17-26. 2018-11-20 [PMID: 30525067] (Bioassay, Human)
• LD Loozen, A Vanderstee, AH Kragten, FC Öner, WJ Dhert, MC Kruyt, J Alblas Bone formation by heterodimers through non-viral gene delivery of BMP-2/6 and BMP-2/7 Eur Cell Mater, 2018-03-28;35(0):195-208. 2018-03-28 [PMID: 29589649] (ELISA (Standard))

Bioinformatics

• Gene Symbol: BMP2
• Uniprot:
  • P12643 (BMP-2) & P22004 (BMP-6)()