Recombinant Human beta-NGF Protein, CF

Recombinant Human beta -NGF (Catalog # 256‑GF/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2-2 ng/mL.

1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human beta-NGF Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED 50 for this effect is 0.2-2 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human beta-NGF protein Ser122-Ala241
• Accession #: CAA36832
• N-terminal Sequence: Ser122
• Protein/Peptide Type: Recombinant Proteins
• Gene: NGF
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 13 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human beta-NGF Protein, CF

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and gamma . Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity. NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Publications for beta-NGF (256-GF/CF)(49)

Publications using 256-GF/CF

• YH Lai, CY Su, HW Cheng, CY Chu, LB Jeng, CS Chiang, WC Shyu, SY Chen Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy Nature Communications, 2023-01-18;14(1):285. 2023-01-18 [PMID: 36650171] (Bioassay, Human)
• S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay Research in Veterinary Science, 2022-08-22;151(0):156-163. 2022-08-22 [PMID: 36029605] (Control, Horse)
• AK Holzer, I Suciu, C Karreman, T Goj, M Leist Specific Attenuation of Purinergic Signaling during Bortezomib-Induced Peripheral Neuropathy In Vitro International Journal of Molecular Sciences, 2022-03-29;23(7):. 2022-03-29 [PMID: 35409095] (Differentiation, Differentiation, Human)
• D Segelcke, HK Fischer, M Hütte, S Dennerlein, F Benseler, N Brose, EM Pogatzki-Z, M Schmidt Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice Cell Reports, 2021-12-21;37(12):110152. 2021-12-21 [PMID: 34936870] (Bioassay, Mouse)
• P Göttle, K Schichel, L Reiche, L Werner, A Zink, A Prigione, P Küry TLR4 Associated Signaling Disrupters as a New Means to Overcome HERV-W Envelope-Mediated Myelination Deficits Frontiers in Cellular Neuroscience, 2021-11-23;15(0):777542. 2021-11-23 [PMID: 34887730] (Bioassay, Rat)
• AT Regua, NR Aguayo, SA Jalboush, DL Doheny, SG Manore, D Zhu, GL Wong, A Arrigo, CJ Wagner, Y Yu, A Thomas, MD Chan, J Ruiz, G Jin, R Strowd, P Sun, J Lin, HW Lo TrkA Interacts with and Phosphorylates STAT3 to Enhance Gene Transcription and Promote Breast Cancer Stem Cells in Triple-Negative and HER2-Enriched Breast Cancers Cancers, 2021-05-12;13(10):. 2021-05-12 [PMID: 34066153] (Bioassay, Human)
• B Sun, X Yang, F Hou, X Yu, Q Wang, HS Oh, P Raja, JM Pesola, EAH Vanni, S McCarron, J Morris-Lov, AHM Ng, GM Church, DM Knipe, DM Coen, D Pan Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency Nature Microbiology, 2021-02-08;0(0):. 2021-02-08 [PMID: 33558653] (Bioassay, Mouse)
• KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells&#039; Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020-08-21;9(9):. 2020-08-21 [PMID: 32839392] (Bioassay, Cell Culture, Human)
• B Xia, J Gao, S Li, L Huang, L Zhu, T Ma, L Zhao, Y Yang, K Luo, X Shi, L Mei, H Zhang, Y Zheng, L Lu, Z Luo, J Huang Mechanical stimulation of Schwann cells promote peripheral nerve regeneration via extracellular vesicle-mediated transfer of microRNA 23b-3p Theranostics, 2020-07-11;10(20):8974-8995. 2020-07-11 [PMID: 32802175] (Bioassay, Cell Culture, Rat)
• H Yu, W Hu, X Song, Y Zhao Generation of Multipotent Stem Cells from Adult Human Peripheral Blood Following the Treatment with Platelet-Derived Mitochondria Cells, 2020-05-29;9(6):. 2020-05-29 [PMID: 32485922] (Bioassay, Human)
• A Yoshida, K Takashima, T Shimonaga, M Kadokura, S Nagase, S Koda Establishment of a simple one-step method for oligodendrocyte progenitor cell preparation from rodent brains J. Neurosci. Methods, 2020-05-30;0(0):108798. 2020-05-30 [PMID: 32479973] (Bioassay, Rat)
• JS Ebo, JC Saunders, PWA Devine, AM Gordon, AS Warwick, B Schiffrin, SE Chin, E England, JD Button, C Lloyd, NJ Bond, AE Ashcroft, SE Radford, DC Lowe, DJ Brockwell An in vivo platform to select and evolve aggregation-resistant proteins Nat Commun, 2020-04-14;11(1):1816. 2020-04-14 [PMID: 32286330] (Bioassay, Human)
• N Uesato, N Miyagawa, K Inagaki, R Kakefuda, Y Kitagawa, Y Matsuo, T Yamaguchi, T Hata, K Ikegashira, M Matsushita Pharmacological Properties of JTE-952, an Orally Available and Selective Colony Stimulating Factor 1 Receptor Kinase Inhibitor Biol. Pharm. Bull., 2020-01-01;43(2):325-333. 2020-01-01 [PMID: 32009119] (Cell Culture, Human)
• AR Nickolls, MM Lee, DF Espinoza, M Szczot, RM Lam, Q Wang, J Beers, J Zou, MQ Nguyen, HJ Solinski, AA AlJanahi, KR Johnson, ME Ward, AT Chesler, CG Bönnemann Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell Rep, 2020-01-21;30(3):932-946.e7. 2020-01-21 [PMID: 31968264] (Differentiation, Differentiation, Human)
• B Xia, J Gao, S Li, L Huang, T Ma, L Zhao, Y Yang, J Huang, Z Luo Extracellular Vesicles Derived From Olfactory Ensheathing Cells Promote Peripheral Nerve Regeneration in Rats Front Cell Neurosci, 2019-12-06;13(0):548. 2019-12-06 [PMID: 31866834] (Bioassay, Rat)
• EP McCurdy, KM Chung, CR Benitez-Ag, U Hengst Promotion of Axon Growth by the Secreted End of a Transcription Factor Cell Rep, 2019-10-08;29(2):363-377.e5. 2019-10-08 [PMID: 31597097] (Bioassay, Rat)
• D Baumeister, W Eich, S Saft, O Geisel, R Hellweg, A Finn, CI Svensson, J Tesarz No evidence for altered plasma NGF and BDNF levels in fibromyalgia patients Sci Rep, 2019-09-20;9(1):13667. 2019-09-20 [PMID: 31541132] (ELISA Standard, Human)
• S Park, JY Kim, S Myung, N Jung, Y Choi, SC Jung Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation Int J Mol Sci, 2019-06-01;20(11):. 2019-06-01 [PMID: 31159418] (Bioassay, Human)
• A Kizilyer, MV Singh, VB Singh, S Suwunnakor, J Palis, SB Maggirwar Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis Sci Rep, 2019-02-26;9(1):2781. 2019-02-26 [PMID: 30808933] (Bioassay, Human)
• AJ Poole, L Frigotto, ME Smith, C Baar, G Ivanova-Be, A Jaulent, C Stace, CG Ullman, AV Hine A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics Sci Rep, 2019-01-30;9(1):930. 2019-01-30 [PMID: 30700786] (ELISA (Capture), ELISA (Standard), Human)
• PM Deardorff, TB McKay, S Wang, CE Ghezzi, DM Cairns, RD Abbott, JL Funderburg, KR Kenyon, DL Kaplan Modeling Diabetic Corneal Neuropathy in a 3D In Vitro Cornea System Sci Rep, 2018-11-23;8(1):17294. 2018-11-23 [PMID: 30470798] (Bioassay, Human)
• K Kirino, T Nakahata, T Taguchi, MK Saito Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition Sci Rep, 2018-08-27;8(1):12865. 2018-08-27 [PMID: 30150715] (Bioassay, Human)
• T Miladinovi, RG Ungard, K Linher-Mel, S Popovic, G Singh Functional effects of TrkA inhibition on system xC--mediated glutamate release and cancer-induced bone pain Mol Pain, 2018-01-01;14(0):1744806918776. 2018-01-01 [PMID: 29761734] (Bioassay, Human)
• DR Gomez, LA Byers, M Nilsson, L Diao, J Wang, L Li, P Tong, M Hofstad, B Saigal, I Wistuba, N Kalhor, S Swisher, Y Fan, WK Hong, M Suraokar, C Behrens, C Moran, JV Heymach Integrative proteomic and transcriptomic analysis provides evidence for TrkB (NTRK2) as a therapeutic target in combination with tyrosine kinase inhibitors for non-small cell lung cancer Oncotarget, 2018-01-30;9(18):14268-14284. 2018-01-30 [PMID: 29581842] (Bioassay, Human)
• JW Martin, JC Chen, J Neidleman, K Tatsumi, J Hu, LC Giudice, WC Greene, NR Roan Potent and rapid activation of tropomyosin-receptor kinase A in endometrial stromal fibroblasts by seminal plasma Biol. Reprod., 2018-08-01;0(0):. 2018-08-01 [PMID: 29518187] (Bioassay, Human)
• KC Chun, SH Lee, JW Kim, EJ Jin, KM Kim, CH Chun Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts J Orthop Surg Res, 2017-06-14;12(1):93. 2017-06-14 [PMID: 28615040] (Bioassay, Human)
• Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo Sci Rep, 2016-12-20;6(0):38644. 2016-12-20 [PMID: 27995962] (Bioassay, Rat)
• Nerve Growth Factor Attenuates Apoptosis and Inflammation in the Diabetic Cornea Invest. Ophthalmol. Vis. Sci, 2016-12-01;57(15):6767-6775. 2016-12-01 [PMID: 27978558] (Bioassay, Human)
• Hierarchical CRMP2 posttranslational modifications control NaV1.7 function Proc. Natl. Acad. Sci. U.S.A, 2016-12-08;0(0):. 2016-12-08 [PMID: 27940916] (Bioassay, Human)
• BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer&#039;s disease models Sci Rep, 2016-09-19;6(0):33427. 2016-09-19 [PMID: 27641902] (Bioassay, Human)
• S Delbary-Go, S Lee, M Baroni, I Lamarche, M Arnone, B Canolle, A Lin, J Sacramento, EA Salegio, MN Castel, N Delesque-T, A Alam, P Laboudie, B Ferzaz, P Savi, JM Herbert, GT Manley, AR Ferguson, JC Bresnahan, F Bono, MS Beattie A novel inhibitor of p75-neurotrophin receptor improves functional outcomes in two models of traumatic brain injury Brain, 2016-04-15;0(0):. 2016-04-15 [PMID: 27084575] (Bioassay, Human)
• Margriet A Huisman Isolation, expansion and neural differentiation of stem cells from human plucked hair: a further step towards autologous nerve recovery Cytotechnology, 2015-12-24;68(5):1849-58. 2015-12-24 [PMID: 26702932] (Bioassay, Human)
• Park K, Lin C, Li K, Lee Y Effects of Reducing Suppressors of Cytokine Signaling-3 (SOCS3) Expression on Dendritic Outgrowth and Demyelination after Spinal Cord Injury. PLoS ONE, 2015-09-18;10(9):e0138301. 2015-09-18 [PMID: 26384335] (Bioassay, Mouse)
• Schwab , Andrew J, Ebert , Allison Sensory neurons do not induce motor neuron loss in a human stem cell model of spinal muscular atrophy. PLoS ONE, 2014-07-23;9(7):e103112. 2014-07-23 [PMID: 25054590] (Bioassay, Human)
• Wan C, Liu J, Nie X, Zhao J, Zhou S, Duan Z, Tang C, Liang L, Xu G 2, 3, 7, 8-Tetrachlorodibenzo-P-dioxin (TCDD) induces premature senescence in human and rodent neuronal cells via ROS-dependent mechanisms. PLoS ONE, 2014-02-24;9(2):e89811. 2014-02-24 [PMID: 24587053] (Bioassay, Rat)
• Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury. Stem Cells Transl Med, 2014-01-17;3(3):334-45. 2014-01-17 [PMID: 24443004] (Bioassay, Canine)
• Ko J, Mizuno Y, Ohki C, Chikama T, Sonoda K, Kiuchi Y Neuropeptides released from trigeminal neurons promote the stratification of human corneal epithelial cells. Invest Ophthalmol Vis Sci, 2014-01-07;55(1):125-33. 2014-01-07 [PMID: 24327615] (Bioassay, Human)
• Bowers P, Neben T, Tomlinson G, Dalton J, Altobell L, Zhang X, Macomber J, Wu B, Toobian R, McConnell A, Verdino P, Chau B, Horlick R, King D Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation. J Biol Chem, 2013-01-25;288(11):7688-96. 2013-01-25 [PMID: 23355464] (Bioassay, Rat)
• Delcroix GJ, Curtis KM, Schiller PC, Montero-Menei CN EGF and bFGF pre-treatment enhances neural specification and the response to neuronal commitment of MIAMI cells. Differentiation, 2010-09-01;80(4):213-27. 2010-09-01 [PMID: 20813449] (Bioassay, Human)
• Fjord-Larsen L, Kusk P, Tornoe J, Juliusson B, Torp M, Bjarkam CR, Nielsen MS, Handberg A, Sorensen JC, Wahlberg LU Long-term delivery of nerve growth factor by encapsulated cell biodelivery in the Gottingen minipig basal forebrain. Mol. Ther., 2010-07-27;18(12):2164-72. 2010-07-27 [PMID: 20664524] (Bioassay, Porcine, Rat)
• Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (ELISA (Standard))
• Foveau B, Ancot F, Leroy C, Petrelli A, Reiss K, Vingtdeux V, Giordano S, Fafeur V, Tulasne D Down-regulation of the met receptor tyrosine kinase by presenilin-dependent regulated intramembrane proteolysis. Mol. Biol. Cell, 2009-03-18;20(9):2495-507. 2009-03-18 [PMID: 19297528] (Bioassay, Canine)
• Samah B, Porcheray F, Gras G Neurotrophins modulate monocyte chemotaxis without affecting macrophage function. Clin. Exp. Immunol., 2008-01-08;151(3):476-86. 2008-01-08 [PMID: 18190610] (Bioassay, Human)
• Paredes D, Granholm AC, Bickford PC Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat. Brain Res., 2007-01-12;1141(0):56-64. 2007-01-12 [PMID: 17292337] (In Vivo, Rat)
• Gallo G RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction. J. Cell. Sci., 2006-08-15;119(0):3413-23. 2006-08-15 [PMID: 16899819] (Bioassay, Chicken)
• Young KR, Ross TM Elicitation of immunity to HIV type 1 Gag is determined by Gag structure. AIDS Res. Hum. Retroviruses, 2006-01-01;22(1):99-108. 2006-01-01 [PMID: 16438652] (Western Blot)
• Whittlesey KJ, Shea LD Nerve growth factor expression by PLG-mediated lipofection. Biomaterials, 2005-11-28;27(11):2477-86. 2005-11-28 [PMID: 16316681] (Western Blot)
• Adams DN, Kao EY, Hypolite CL, Distefano MD, Hu WS, Letourneau PC Growth cones turn and migrate up an immobilized gradient of the laminin IKVAV peptide. J. Neurobiol., 2005-01-01;62(1):134-47. 2005-01-01 [PMID: 15452851] (Bioassay, Chicken)
• Dolle L, El Yazidi-Belkoura I, Adriaenssens E, Nurcombe V, Hondermarck H Nerve growth factor overexpression and autocrine loop in breast cancer cells. Oncogene, 2003-08-28;22(36):5592-601. 2003-08-28 [PMID: 12944907] (Bioassay, ELISA (Standard), Chicken)

Bioinformatics

• Gene Symbol: NGF
• Uniprot:
  • CAA36832()