Recombinant Human B7-H2 His-tag Protein, CF

Recombinant Human B7-H2 co-stimulates IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.

1 μg/lane ofRecombinant Human B7-H2 (Catalog # 8206-B7) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing bands at 64.3 and 64.4 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human B7-H2 His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.3-1.5 μg/mL. Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808. The ED₅₀ for this effect is 0.3-1.2 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human B7-H2 protein
  Asp19-Ser258, with a C-terminal 6-His tag
• Accession #: O75144
• N-terminal Sequence: Asp19
• Protein/Peptide Type: Recombinant Proteins
• Gene: ICOSLG
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 59-64 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human B7-H2 His-tag Protein, CF

• B7H2
• B7-H2
• B7H2B7 homolog 2
• B7-H2B7-related protein 1
• B7RP-1
• B7RP1B7-like protein Gl50
• B7RP-1LICOS
• CD275 antigen
• CD275
• GL50
• GL50transmembrane protein B7-H2 ICOS ligand
• ICOSL
• ICOS-L
• ICOSLG
• inducible T-cell co-stimulator ligand
• KIAA0653ICOS ligand

Background

B7-H2, also known as B7-related protein (B7RP1), ICOS Ligand, and CD275, is an approximately 60 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature human B7-H2 consists of a 238 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 25 aa cytoplasmic domain (2, 3). Within the ECD, human B7-H2 shares 50% and 54% aa sequence identity with mouse and rat B7‑H2, respectively. Alternative splicing generates a long isoform that carries a 10 aa substitution for the C-terminal 3 residues. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells (2-6). It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation (2, 4, 7). Mouse and human B7-H2 exhibit cross-species binding to ICOS (3, 6). The B7-H2 interaction with ICOS is co-stimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (Tfh) and germinal centers (2-4, 8, 9). In human but not in mouse, B7-H2 additionally binds to CD28 and CTLA4, and its interaction with CD28 can co-stimulate both human and mouse naïve T cells and regulatory T cells (Treg) (6). B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung (8, 10, 11). Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity (5, 12, 13). In contrast, its ligation of ICOS on Tfh cells can increase the severity of autoimmune symptoms (9). A soluble form of human B7-H2 is elevated in the circulation of patients with active systemic lupus erythematosus (14).In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones (15).

1. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
2. Wang, S. et al. (2000) Blood 96:2808.
3. Yoshinaga, S.K. et al. (2000) Int. Immunol. 12:1439.
4. Yoshinaga, S.K. et al. (1999) Nature 402:827.
5. Faget, J. et al. (2012) Cancer Res. 72:6130.
6. Yao, S. et al. (2011) Immunity 32:729.
7. Watanabe, M. et al. (2008) J. Immunol. 180:5222.
8. Wong, S.-C. et al. (2003) Blood 102:1381.
9. Hu, Y.-L. et al. (2009) J. Immunol. 182:1421.
10. Kadkhoda, K. et al. (2010) J. Immunol. 184:3780.
11. Kadkhoda, K. et al. (2011) Int. Immunol. 23:239.
12. Gajewska, B.U. et al. (2005) J. Immunol. 174:3000.
13. Akbari, O. et al. (2002) Nat. Med. 8:1024.
14. Her, M. et al. (2009) Lupus 18:501.
15. Wang, F. et al. (2012) J. Clin. Immunol. 32:1253.

Bioinformatics

• Gene Symbol: ICOSLG
• Uniprot:
  • O75144()