Recombinant Human Azurocidin/CAP37/HBP Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Azurocidin/CAP37/HBP Protein Summary

Details of Functionality
Measured by its ability to enhance LPS-induced TNF-alpha secretion from human monocytes. Rasmussen, P.B. et al. (1996) FEBS Letters 390:109. The ED50 for this effect is 0.5-3 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Azurocidin/CAP37/HBP protein
Ile27-Pro250, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ile27
Protein/Peptide Type
Recombinant Enzymes
Gene
AZU1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
39 kDa, reducing conditions
Publications
Read Publications using
2200-SE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Azurocidin/CAP37/HBP Protein

  • AZAMP
  • AZU
  • AZU1
  • azurocidin 1
  • Azurocidin
  • CAP37
  • CAP37Heparin-binding protein
  • cationic antimicrobial protein 37
  • HBP
  • HBPCationic antimicrobial protein CAP37
  • HUMAZUR
  • NAZC
  • neutrophil azurocidin

Background

Azurocidin, also known as cationic antimicrobial protein 37 (CAP37) and heparin-binding protein (HBP), is a member of the serine protease family that includes Cathepsin G, neutrophil elastase (NE), and proteinase 3 (PR3). These proteases are found in the specialized azurophilic granules of neutrophils (1, 2). Human Azurocidin 1 is encoded by the AZU1 gene located in a cluster with NE and PR3 on chromosome 19pter (2). The open reading frame predicts a 251 amino acid (aa) protein with an N-terminal 26 aa signal sequence and a 7 aa propeptide. There are also eight cysteine residues and 3 putative N-linked glycosylation sites (1).

Although Azurocidin 1 shares a significant degree of aa sequence identity with Cathepsin G, NE, and PR3, it lacks serine protease activity due to mutations at two of the three residues in the catalytic triad (His41Ser and Ser175Gly) (1, 3). Crystallographic analysis suggests that the antibacterial activity of Azurocidin is mediated by a hydrophobic pocket (residues 20 to 44) that binds Gram-negative bacteria lipid A. These structural data also imply that the heparin binding capacity is mediated by non-specific electrostatic interactions between the negatively charged heparin molecule and a large patch of positively charged residues near the lipid A binding site (3).

Azurocidin has also been identified as a modulator of endothelial permeability. Neutrophils arriving first at sites of inflammation release Azurocidin, which acts in a paracrine fashion on endothelial cells causing the development of intercellular gaps and allowing leukocyte extravasation. These findings imply that Azurocidin may be a reasonable therapeutic target for a variety of inflammatory disease conditions (4).

  1. Morgan, J.G. et al. (1991) J. Immunol. 147:3210.
  2. Zimmer, M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8215.
  3. Iverson, L.F. et al. (1997) Nat. Struct. Biol. 4:265.
  4. Gautam, N. et al. (2001) Nat. Med. 7:1123.

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Publications for Azurocidin/CAP37/HBP (2200-SE)(3)

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Bioinformatics

Gene Symbol AZU1
Uniprot