Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein, CF Summary
Additional Information |
Biotinylated (HEK293-expressed) |
Details of Functionality |
Measured by its binding ability in a functional ELISA. When
Recombinant Human CD6 Fc Chimera (Catalog #
627-CD) is immobilized at 0.5 µg/mL
(100 µL/well), Biotinylated Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag (Catalog
# AVI7187) binds with an ED 50 of 1.50-12.0 ng/mL. |
Source |
Human embryonic kidney cell, HEK293-derived human ALCAM/CD166 protein Human ALCAM (Trp28-Ala526) Accession # AAB59499.1 | DIEGRMD | Human IgG1 (Pro100-Lys330) | Avi-tag | N-terminus | | | C-terminus | |
|
N-terminal Sequence |
Trp28 |
Structure / Form |
Disulfide-linked homodimer Biotinylated via Avi-tag |
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
84 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
100-120 kDa, under reducing conditions. |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Purity |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 250 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human ALCAM/CD166 Fc Chimera Avi-tag Protein, CF
Background
Activated
leukocyte cell adhesion molecule (ALCAM), also known as CD166, is a surface glycoprotein
belonging to the immunoglobulin superfamily (1, 2). ALCAM, along with MCAM and
BCAM/Lu, form a small subgroup of adhesion molecules involved in tissue
development and maintenance, neurogenesis, and regulation of immune responses (3).
Mature human ALCAM consists of an extracellular domain (ECD) containing 2 IgV
and 3 IgC domains, a transmembrane domain, and a short cytoplasmic domain (1, 2).
Within the ECD, human ALCAM shares 93% amino acid sequence identity with both
mouse and rat ALCAM. An isoform of ALCAM with a shorter stalk region in the ECD
is known to exist and is associated with higher shedding tendency and decreased
cell adhesion (2). Initially found expressed on activated leukocytes, ALCAM expression
has been detected in a wide variety of tissues and cells (3, 4). In addition to
cell adhesion, other ALCAM functions include leukocyte migration across the
blood brain barrier, T-cell activation, and osteogenesis, (2, 5, 6). Overexpression
of ALCAM is often associated with poor prognosis in various human tumors, such
as bladder cancer, prostate cancer, melanoma, and liver cancer (2, 7). Our Avi-tag Biotinylated Recombinant
Human
ALCAM features biotinylation at a single site
contained within the Avi-tag, a unique 15 amino acid peptide. Protein
orientation will be uniform when bound to streptavidin-coated surface due to
the precise control of biotinylation and the rest of the protein is unchanged
so there is no interference in the protein's bioactivity.
- Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
- Ferragut, F. et al. (2021) Cytokine Growth Factor Rev. 61:27.
- Swart, G.W. (2002) Eur J Cell Biol. 81:313).
- Zimmerman, A.W. et al. (2006) Blood 107:3212.
- Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
- Cayrol, R. et al. (2008) Nat. Immunol. 9:137.
- Darvishi, B. et al. (2020) Exp. & Mol. Path 115:104443.
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