Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF


There are currently no images for alpha-Galactosidase/a-Gal (6415-GH).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity EcSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF Summary

Details of Functionality
Measured by its ability to hydrolyze 4-methylumbelliferyl-alpha -D-galactopyranoside. The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.
E. coli-derived e. coli alpha-Galactosidase/a-Gal protein
Val2-Val708, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
70-75 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Assay Procedure
  • Assay Buffer: 100 mM MES, pH 6.5
  • Recombinant E. coli alpha ‑Galactosidase/ alpha ‑Gal (rE. coli alpha -Gal) (Catalog # 6415-GH)
  • Substrate: 4-Methylumbelliferyl-alpha -D-galactopyranoside (Sigma, Catalog # M7633), 6.7 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rE. coli alpha -Gal to 0.5 ng/μL in Assay Buffer.
  2. Dilute Substrate to 800 μM in Assay Buffer.
  3. Load into a plate 50 μL of 0.5 ng/μL rE. coli alpha -Gal, and start the reaction by adding 50 μL of 800 μM Substrate. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of 800 μM Substrate.
  4. Read plate at excitation and emission wavelengths of 365 nm and 445 nm, respectively, in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 4-Methylumbelliferone (4-MU) (Sigma, Catalog # M1381).

Per Well:
  • rE. coli alpha -Gal: 0.025 μg
  • Substrate: 400 μM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF

  • alphaGalactosidase
  • alpha-Galactosidase
  • rafA


alpha -Galactoside is prevalent in animals and plants. The alpha -Galactosidase ( alpha -Gal) from Escherichia coli is a useful tool for the removal of alpha 1,3-linked and alpha 1,6‑linked galactosides from the non-reducing terminus of complex carbohydrates and glycoproteins (1). alpha -Gal efficiently hydrolyzes raffinose, an alpha -D-galactosylsucrose, to D‑galactose and sucrose. The enzyme can also hydrolyze other alpha -Galactosides such as melibiose, stachyose, verbascose, and galactinol. The enzyme does not cleave beta -linked galactose, such as lactose.
  1. Schmid, K. and Schmitt, R. (1976) Eur. J. Biochem. 67:95.
  2. Spangenberg, P. et al. (2000) Carbohydr. Res. 329:65.

Publications for alpha-Galactosidase/a-Gal (6415-GH) (0)

There are no publications for alpha-Galactosidase/a-Gal (6415-GH).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for alpha-Galactosidase/a-Gal (6415-GH) (0)

There are no reviews for alpha-Galactosidase/a-Gal (6415-GH). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for alpha-Galactosidase/a-Gal (6415-GH) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional alpha-Galactosidase/a-Gal Products

Bioinformatics Tool for alpha-Galactosidase/a-Gal (6415-GH)

Discover related pathways, diseases and genes to alpha-Galactosidase/a-Gal (6415-GH). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on alpha-Galactosidase/a-Gal

There are no specific blogs for alpha-Galactosidase/a-Gal, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant E. coli alpha-Galactosidase/a-Gal Protein, CF and receive a gift card or discount.


Gene Symbol rafA