Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

When Recombinant Human IL-21 Protein (8879-IL) is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera (10482-R2) that produces a 50% optimal binding response is found to be 0.6-3.6 μg/mL.

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera Protein (Catalog # 10482-R2) was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® Blue staining, showing bands at kDa.

• Reactivity: Pm-Cm, RM
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-21 Protein (Catalog # 8879-IL) is immoblized at 2.5 µg/mL (100 µL/well), the concentration of Recombinant Cynomolgus Monkey/Rhesus Macaque IL-21R Fc Chimera (Catalog # 10482-R2) that produces a 50% optimal binding response is found to be 0.6-3.6 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived IL-21R protein
  

  Cynomolgus Monkey/Rhesus Macaque IL‑21R (Cys20-Pro236) Accession # XP_005591578.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: XP_005591578.1
• N-terminal Sequence: Cys20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-80 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus/Rhesus IL-21R Fc Chimera Protein, CF

• CD360 antigen
• CD360
• IL-21 R
• IL-21 receptor
• IL21R
• IL-21R
• interleukin 21 receptor
• interleukin-21 receptor
• MGC10967
• NILR
• Novel interleukin receptor

Background

Interleukin-21 Receptor (IL-21 R) is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common  gamma  chain ( gamma _c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 (2, 3). Based on its similarity with human IL-21 R, mature cynomolgus/rhesus IL-21 R is predicted to consist of a 213 amino acid (aa) extracellular domain (ECD), a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 253 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, cynomolgus/rhesus IL-21 R shares 98% aa identity with human IL-21 R. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8⁺ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15).

1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
2. Asao, H. et al. (2001) J. Immunol. 167:1.
3. Habib, T. et al. (2002) Biochemistry 41:8725.
4. Parrish-Novak, et al. (2000) Nature 408:57.
5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
6. Ozaki, K. et al. (2002) Science 298:1630.
7. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
8. King, I.L. et al. (2010) J. Immunol. 185:6138.
9. Kasaian, M.T. et al. (2002) Immunity 16:559.
10. Frohlich, A. et al. (2009 Science 324:1576.
11. Tortola, L. et al. (2010) Blood 116:5200.
12. Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.
13. Moser, E.K. et al. (2015) PLoS One 10:e0120169.
14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
15. Sarosiek, K.A. et al. (2010) Blood 115:570.

Bioinformatics

• Uniprot:
  • XP_005591578.1()