When Recombinant Cynomolgus Monkey ALCAM (Catalog # 10027-AL) is coated onto a microplate at 1 µg/mL, Biotinylated Recombinant Human CD6 Fc Chimera binds withan ED50 of 10-50 ng/mL.
2 μg/lane of Recombinant Cynomolgus Monkey ALCAM/CD166 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-89 kDa.
Measured by its binding ability in a functional ELISA. When Recombinant Human Cynomolgus Monkey ALCAM/CD166 is immobilized at 1 µg/mL (100
µL/well), Biotinylated Recombinant Human CD6 Fc Chimera binds with an ED50 of 10-50 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey ALCAM/CD166 protein Trp28-Ala526, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
67-89 kDa, reducing conditions
Publications
Read Publication using 10027-AL in the following applications:
ALCAM
(activated leukocyte cell adhesion molecule), designated CD166, is a 100‑110 kDa type I transmembrane glycoprotein and a
member of the Ig CAM family within the immunoglobulin superfamily (1). The
cynomologous ALCAM amino acid (aa) sequence includes a signal peptide, an extracellular
domain (ECD) with two V‑type and three C2‑type Ig‑like domains, a transmembrane domain and a short
cytoplasmic domain (1). Human ALCAM has several isoforms, including an isoform lacking
most of the cytoplasmic domain and a secreted isoform (sALCAM) which antagonizes
full‑length
ALCAM (2, 3). Mature cynomologous ALCAM ECD shares 93% and 96% aa sequence
identity with human and mouse/rat ALCAM, respectively. ALCAM is expressed on multiple cell types
including thymic epithelium, microvascular endothelium, activated lymphocytes
and monocytes, and monocyte‑derived dendritic cells (1, 4). ALCAM mediates
low‑affinity
adhesion with itself or the cysteine‑rich scavenger receptor CD6 to regulate T cell
development, immunological synapses (IS), and cell migration through
endothelial junctions (1‑11). ALCAM on thymic epithelia mediates adhesion
to CD6 on CD4+CD8+ T cells (6). Adhesion of ALCAM‑expressing antigen presenting cells and CD6‑expressing T cells stabilizes the early IS,
while later it enhances CD3 effects on T cell proliferation, CD25 expression,
and Th1 commitment (4, 7, 8). High ALCAM expression at the blood‑brain barrier in active multiple sclerosis, and
its mouse model (EAE), promotes leukocyte migration to the brain (8, 9). High ALCAM
expression on melanoma cell lines appears to be pro‑metastatic, but anti‑metastatic activity has been reported in breast
cancer (3, 10, 11). ALCAM may influence expression or adhesion of the neuronal
adhesion molecule NCAM‑L1, both in the developing retina and invasive
melanoma (2, 12).
Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
van Kilsdonk, J.W.J. et al. (2008) Cancer Res. 68:3671.
Ikeda, K. and T. Quertermous (2004) J. Biol. Chem. 279:55315.
Zimmerman, A.W. et al. (2006) Blood 107:3212.
van Kempen, L.C. et al. (2001) J. Biol. Chem. 276:25783.
Castro, M.A.A. et al. (2007) J. Immunol. 178:4351.
Nair, P. et al. (2010) Clin. Exp. Immunol. 162:116.
Masedunskas, A. et al. (2006) FEBS Lett. 580:2637.
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