Recombinant Canine IL-4 Protein, CF

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Canine IL-4 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 3‑18 ng/mL.
Source
E. coli-derived canine IL-4 protein
His25-His132, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
754-CL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Canine IL-4 Protein, CF

  • B cell growth factor 1
  • BCDF
  • B-cell stimulatory factor 1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cell stimulatory factor 1
  • interleukin 4
  • interleukin-4
  • Lymphocyte stimulatory factor 1
  • MGC79402
  • pitrakinra

Background

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1 - 3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Canine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature canine IL-4 shares 81%, 61%, 46%, 38%, and 37% aa sequence identity with feline, bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8-11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Khatlani, T. et al. (1999) J. Vet. Med. Sci. 61:967.
  4. van der Kaaij, S.Y. et al. (1999) Immunogenetics 49:142.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

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Publications for IL-4 (754-CL/CF)(11)

We have publications tested in 2 confirmed species: Canine, N/A.

We have publications tested in 2 applications: Bioassay, ELISA Standard.


Filter By Application
Bioassay
(5)
ELISA Standard
(6)
All Applications
Filter By Species
Canine
(8)
N/A
(3)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 754-CL/CF Applications Species
F Heinrich, A Lehmbecker, BB Raddatz, K Kegler, A Tipold, VM Stein, A Kalkuhl, U Deschl, W Baumgärtne, R Ulrich, I Spitzbarth Morphologic, phenotypic, and transcriptomic characterization of classically and alternatively activated canine blood-derived macrophages in vitro PLoS ONE, 2017;12(8):e0183572. 2017 [PMID: 28817687] (Bioassay, Canine) Bioassay Canine
Impact of LbSapSal Vaccine in Canine Immunological and Parasitological Features before and after Leishmania chagasi-Challenge PLoS ONE, 2016;11(8):e0161169. 2016 [PMID: 27556586] (ELISA Standard, Canine) ELISA Standard Canine
Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs PLoS Negl Trop Dis, 2016;10(5):e0004614. 2016 [PMID: 27223609] (ELISA Standard, Canine) ELISA Standard Canine
Costa-Pereira C, Moreira M, Soares R, Marteleto B, Ribeiro V, Franca-Dias M, Cardoso L, Viana K, Giunchetti R, Martins-Filho O, Araujo M One-year timeline kinetics of cytokine-mediated cellular immunity in dogs vaccinated against visceral leishmaniasis. BMC Vet Res, 2015;11(0):92. 2015 [PMID: 25880646] (ELISA Standard, Canine) ELISA Standard Canine
Qeska V, Barthel Y, Herder V, Stein V, Tipold A, Urhausen C, Gunzel-Apel A, Rohn K, Baumgartner W, Beineke A Canine distemper virus infection leads to an inhibitory phenotype of monocyte-derived dendritic cells in vitro with reduced expression of co-stimulatory molecules and increased interleukin-10 transcription. PLoS ONE, 2014;9(4):e96121. 2014 [PMID: 24769532] (Bioassay, Canine) Bioassay Canine
Xiong W, Candolfi M, Liu C Human Flt3L generates dendritic cells from canine peripheral blood precursors: implications for a dog glioma clinical trial. PLoS ONE, 2010;5(6):e11074. 2010 [PMID: 20552015] (Bioassay, Canine) Bioassay Canine
Lange S, Altmann S, Brandt B, Adam C, Riebau F, Vogel H, Weirich V, Hilgendorf I, Storb R, Freund M, Junghanss C Investigation of immunological approaches to enhance engraftment in a 1 Gy TBI canine hematopoietic stem cell transplantation model. Exp. Hematol., 2009;37(1):143-50. 2009 [PMID: 19100524] (Bioassay, Canine) Bioassay Canine
Mason NJ, Coughlin CM, Overley B, Cohen JN, Mitchell EL, Colligon TA, Clifford CA, Zurbriggen A, Sorenmo KU, Vonderheide RH RNA-loaded CD40-activated B cells stimulate antigen-specific T-cell responses in dogs with spontaneous lymphoma. Gene Ther., 2008;15(13):955-65. 2008 [PMID: 18337841] (Bioassay, Canine) Bioassay Canine
Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial. Vaccine, 2007;25(21):4223-34. 2007 [PMID: 17395339] (ELISA Standard, N/A) ELISA Standard N/A
Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs. Vaccine, 2005;24(4):501-9. 2005 [PMID: 16140437] (ELISA Standard, N/A) ELISA Standard N/A
Show All 11 Publications.

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Bioinformatics

Gene Symbol IL4
Entrez
Uniprot