RCC2 Antibody

Images

 
Western Blot: RCC2 Antibody [NBP1-32602] - Sample(30 ug whole cell lysate)A:H1299B:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: RCC2 Antibody [NBP1-32602] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.
Western Blot: RCC2 Antibody [NBP1-32602] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RCC2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RCC2. The exact sequence is proprietary.
Localization
Nucleus, nucleolus, Centromere, Spindle
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RCC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RCC2 Antibody

  • DKFZp762N0610
  • KIAA1470
  • protein RCC2
  • RCC1-like protein TD-60
  • regulator of chromosome condensation 2
  • TD60
  • TD-60
  • Telophase disk protein of 60 kDa

Background

RCC2 (regulator of chromosome condensation 2) is a member of the RCC1 family of guanine nucleotide exchange factors. RCC2 has been identified as part of the chromosomal passenger complex (CPC) which coordinates the chromosomal and cytoskeletal events of mitosis. Chromosomal passengers are proteins present in complexes with aurora B, inner centromeric protein (INCENP), survivin, and borealin. RCC2 has been demonstrated to be required for the recruitment of survivin and aurora B and is essential for proper chromosome segregation and the completion of cell cleavage. Loss of RCC2 inhibits spindle assembly, activates the spindle assembly checkpoint, and blocks cells in prometaphase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional RCC2 Products

RCC2 NBP1-32602

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Bioinformatics

Gene Symbol RCC2