RCC2 Antibody - BSA Free

Western Blot: RCC2 Antibody [NBP1-32602] - KYSE-30 and KYSE-450 human esophageal cancer cells were transfected with a RCC2 over-expression plasmid or mock and RCC2 expression was detected by Western Blot. WB image submitted by a verified customer review.

Immunohistochemistry-Paraffin: RCC2 Antibody [NBP1-32602] - SW480 xenograft, using RCC2 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 mi.

Western Blot: RCC2 Antibody [NBP1-32602] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.

Western Blot: RCC2 Antibody [NBP1-32602] - Sample(30 ug whole cell lysate)A:H1299B:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RCC2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RCC2 Antibody - BSA Free (NBP1-32602) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RCC2. The exact sequence is proprietary.
• Localization: Nucleus, nucleolus, Centromere, Spindle
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RCC2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RCC2 Antibody - BSA Free

• DKFZp762N0610
• KIAA1470
• protein RCC2
• RCC1-like protein TD-60
• regulator of chromosome condensation 2
• TD60
• TD-60
• Telophase disk protein of 60 kDa

Background

RCC2 (regulator of chromosome condensation 2) is a member of the RCC1 family of guanine nucleotide exchange factors. RCC2 has been identified as part of the chromosomal passenger complex (CPC) which coordinates the chromosomal and cytoskeletal events of mitosis. Chromosomal passengers are proteins present in complexes with aurora B, inner centromeric protein (INCENP), survivin, and borealin. RCC2 has been demonstrated to be required for the recruitment of survivin and aurora B and is essential for proper chromosome segregation and the completion of cell cleavage. Loss of RCC2 inhibits spindle assembly, activates the spindle assembly checkpoint, and blocks cells in prometaphase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for RCC2 Antibody (NBP1-32602) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/20/2020

Summary

• Application: Western Blot
• Sample Tested: human esophageal cancer cells
• Species: Human
• Lot: 40002

Bioinformatics

• Gene Symbol: RCC2