RCC2 Antibody

Western Blot: RCC2 Antibody [NB110-40619] - Detection of both input and immunoprecipitated RCC2 on KYSE-30 and KYSE-450 cell lines. Western blot image submitted by a verified customer review.

Immunohistochemistry-Paraffin: RCC2 Antibody [NB110-40619] - RCC2 expression from esophageal cancer tissue from mice. IHC-P image submitted by a verified customer review.

Western Blot: RCC2 Antibody [NB110-40619] - Detection of Human and Mouse RCC2 on HeLa whole cell lysate using NB110-40619. RCC2 was immunoprecipitated by rabbit anti-RCC2 antibody NB110-40618.

Immunohistochemistry-Paraffin: RCC2 Antibody [NB110-40619] - Sections of human skin basal cell carcinoma (left) and mouse CT26 colon carcinoma (right). Affinity purified rabbit anti- RCC2 used at a dilution of 1:1000 (0.2 ug/mL) and 1:200 (1 ug/mL). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RCC2 Antibody Summary

• Description: Novus Biologicals Rabbit RCC2 Antibody (NB110-40619) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-RCC2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 475 and the C-terminus (residue 522) of human Regulator of Chromosome Condensation 2 using the numbering given in entry NP_061185.1 (GeneID 55920).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RCC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. RCC2 antibody validated for IHC-P, WB from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RCC2 Antibody

• DKFZp762N0610
• KIAA1470
• protein RCC2
• RCC1-like protein TD-60
• regulator of chromosome condensation 2
• TD60
• TD-60
• Telophase disk protein of 60 kDa

Background

RCC2 (regulator of chromosome condensation 2) is a member of the RCC1 family of guanine nucleotide exchange factors. RCC2 has been identified as part of the chromosomal passenger complex (CPC) which coordinates the chromosomal and cytoskeletal events of mitosis. Chromosomal passengers are proteins present in complexes with aurora B, inner centromeric protein (INCENP), survivin, and borealin. RCC2 has been demonstrated to be required for the recruitment of survivin and aurora B and is essential for proper chromosome segregation and the completion of cell cleavage. Loss of RCC2 inhibits spindle assembly, activates the spindle assembly checkpoint, and blocks cells in prometaphase. RCC2 is also known as telophase disk protein of 60 kDa, RCC1-like protein TD-60, TD60, KIAA1470, and DKFZp762N0610.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RCC2 Antibody (NB110-40619)(2)

Publications using NB110-40619

• Christian H Bergsland, Jarle Bruun, Marianne G Guren, Aud Svindland, Merete Bjørnslett, Jørgen Smeby, Merete Hektoen, Matthias Kolberg, Enric Domingo, Teijo Pellinen, Ian Tomlinson, David Kerr, David N Church, Arild Nesbakken, Anita Sveen, Ragnhild A Lothe Prediction of relapse-free survival according to adjuvant chemotherapy and regulator of chromosome condensation 2 (RCC2) expression in colorectal cancer ESMO Open 2020-01-01 [PMID: 33219056]
• Bruun J, Kolberg M et al. Regulator of Chromosome Condensation 2 Identifies High-Risk Patients within Both Major Phenotypes of Colorectal Cancer. Clin Cancer Res 2015-08-15 [PMID: 25910952] (IF/IHC, ICC/IF, Human)

Reviews for RCC2 Antibody (NB110-40619) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Ali Calderon Aparicio IHC-P Mouse 11/04/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Esophagus tissue
• Species: Mouse
• Lot: A1

Comments

• Comments: Antigen retrieval with citrate buffer pH 6

reviewed by: Ali Calderon WB Human 03/19/2019

Summary

• Application: Western Blot
• Sample Tested: 293T cell line,Cancer Cell lysate
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: RCC2
• Entrez:
  • 55920(Human)
  • 108911(Mouse)
• Uniprot:
  • NP_061185.1(Human)