RCAN1 Antibody

Immunocytochemistry/Immunofluorescence: RCAN1 Antibody [NBP3-23371] - Immunocytochemical labeling of RCAN1 in aldehyde-fixed NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-RCAN1 (C-terminus) (NBP3-23371) and anti-RCAN1 (a.a. 132-140) (NBP3-23360) antibodies (Left side). These antibodies were also used in the presence (Right side) of blocking peptide RX3945 and RX3965, respectively. The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Western Blot: RCAN1 Antibody [NBP3-23371] - Western blot analysis of RCAN1 expression in human Jurkat (lane 1), rat PC12 (lane 2), human A431 (lane 3), and adult mouse muscle (lane 4). The blot was probed with rabbit polyclonal anti-RCAN1 (C-terminus) at 1:1000.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RCAN1 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: RCAN1 (C-terminus) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminus of human RCAN1. This sequence is highly conserved in rat and mouse RCAN1, and this sequence is found in all four isoforms of RCAN1, but is not found in RCAN2 or RCAN3. [UniProt# P53805]
• Specificity: The antibody detects several isoforms of RCAN1 on SDS-PAGE immunoblots of adult mouse muscle and brain, rat PC12 cells, and human A431 and Jurkat cells. The predominate isoforms detected were the 38 kDa RCAN1L and 31 kDa RCAN1S.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RCAN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for RCAN1 Antibody

• ADAPT78
• calcipressin-1
• calcium and oxidant-inducible mRNA
• Down syndrome candidate region 1
• Down syndrome critical region gene 1
• Down syndrome critical region protein 1
• DSC1
• modulatory calcineurin-interacting protein 1
• Myocyte-enriched calcineurin-interacting protein 1
• near DSCR proline-rich protein
• regulator of calcineurin 1CSP1DSCR1MCIP1RCN1

Background

RCAN1 interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways, possibly affecting central nervous system development. RCAN1 is located in the minimal candidate region for the Down syndrome phenotype, and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Three transcript variants encoding three different isoforms have been found for RCAN1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RCAN1