RBPMS Antibody (OTI2G10) - Azide and BSA Free

Western Blot: RBPMS Antibody (OTI2G10) - Azide and BSA Free [NBP2-73835] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBPMS.

Immunohistochemistry: RBPMS Antibody (OTI2G10) - Azide and BSA Free [NBP2-73835] - Analysis of Human lung tissue.

Flow Cytometry: RBPMS Antibody (OTI2G10) - Azide and BSA Free [NBP2-73835] - Analysis of HEK293T cells transfected with either RC200248 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by RBPMS antibody, and then analyzed by flow cytometry.

Immunohistochemistry: RBPMS Antibody (OTI2G10) - Azide and BSA Free [NBP2-73835] - Analysis of Adenocarcinoma of Human endometrium tissue.

Immunohistochemistry: RBPMS Antibody (OTI2G10) - Azide and BSA Free [NBP2-73835] - Analysis of Carcinoma of Human kidney tissue.

Immunohistochemistry: RBPMS Antibody (OTI2G10) - Azide and BSA Free [NBP2-73835] - Analysis of Human Kidney tissue.

Immunohistochemistry: RBPMS Antibody (OTI2G10) - Azide and BSA Free [NBP2-73835] - Analysis of Human prostate tissue.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI2G10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RBPMS Antibody (OTI2G10) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RBPMS Antibody (OTI2G10) - Azide and BSA Free (NBP2-45551) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-RBPMS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RBPMS (NP_001008712) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBPMS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 24.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RBPMS Antibody (OTI2G10) - Azide and BSA Free

• FLJ32971
• Heart and RRM expressed sequence
• HERMES
• HERMESRNA-binding protein with multiple splicing
• RBPMS
• RBP-MS
• RNA binding protein with multiple splicing

Background

Encoded by a member of the RRM family of RNA-binding proteins, RBPMS is involved in nucleic acid, nucleotide, RNA and protein binding. The RRM family can be found between 80-100 amino acids in length. RBPMS also acts as a coactivator of transcriptional activity and is required for TGFB1/Smad-mediated transactivation. More specifically, RBPMS acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity while promoting the nuclear accumulation of these proteins. Diseases associated with RBPMS include hepatocellular carcinoma, ataxia, carcinoma and neuronitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBPMS Antibody (NBP2-73835)(1)

Publication using NBP2-73835

• Gudiseva HV, Vrathasha V, He J Et al. Single Cell Sequencing of Induced Pluripotent Stem Cell Derived Retinal Ganglion Cells (iPSC-RGC) Reveals Distinct Molecular Signatures and RGC Subtypes Genes (Basel) 2021-12-24 [PMID: 34946963] (FLOW, Human)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.

Bioinformatics

• Gene Symbol: RBPMS