RBPMS Antibody (HL1105) - Azide and BSA Free

Western Blot: RBPMS Antibody (HL1105) [NBP3-13722] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody [HL1105] (NBP3-13722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: RBPMS Antibody (HL1105) [NBP3-13722] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody [HL1105] (NBP3-13722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: RBPMS Antibody (HL1105) [NBP3-13722] - RBPMS antibody [HL1105] detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: RBPMS stained by RBPMS antibody [HL1105] (NBP3-13722) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: RBPMS Antibody (HL1105) [NBP3-13722] - RBPMS antibody [HL1105] detects RBPMS protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: RBPMS stained by RBPMS antibody [HL1105] (NBP3-13722) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: RBPMS Antibody (HL1105) [NBP3-13722] - RBPMS antibody [HL1105] detects RBPMS protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.RBPMS stained by RBPMS antibody [HL1105] (NBP3-13722) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: RBPMS Antibody (HL1105) [NBP3-13722] - RBPMS antibody [HL1105] detects RBPMS protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.RBPMS stained by RBPMS antibody [HL1105] (NBP3-13722) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: RBPMS Antibody (HL1105) [NBP3-13722] - Non-transfected (–) and transfected (+) HCT-116 whole cell extract (60 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RBPMS antibody [HL1105] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: HL1105
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RBPMS Antibody (HL1105) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RBPMS. The exact sequence is proprietary.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RBPMS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for RBPMS Antibody (HL1105) - Azide and BSA Free

• FLJ32971
• Heart and RRM expressed sequence
• HERMES
• HERMESRNA-binding protein with multiple splicing
• RBPMS
• RBP-MS
• RNA binding protein with multiple splicing

Background

Encoded by a member of the RRM family of RNA-binding proteins, RBPMS is involved in nucleic acid, nucleotide, RNA and protein binding. The RRM family can be found between 80-100 amino acids in length. RBPMS also acts as a coactivator of transcriptional activity and is required for TGFB1/Smad-mediated transactivation. More specifically, RBPMS acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity while promoting the nuclear accumulation of these proteins. Diseases associated with RBPMS include hepatocellular carcinoma, ataxia, carcinoma and neuronitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBPMS