RBPMS Antibody (1090828) [Unconjugated]

Western Blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human RBPMS Monoclonal Antibody (Catalog # MAB11670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for RBPMS at approximately 28, 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

RBPMS was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human RBPMS Monoclonal Antibody (Catalog # MAB11670) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm of smooth muscle. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 1090828
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RBPMS Antibody (1090828) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
  Accession # Q93062
• Specificity: Detects a peptide specific for human RBPMS around amino acid 150 in Direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RBPMS Antibody (1090828) [Unconjugated]

• FLJ32971
• Heart and RRM expressed sequence
• HERMES
• HERMESRNA-binding protein with multiple splicing
• RBPMS
• RBP-MS
• RNA binding protein with multiple splicing

Background

RBPMS is a 22kDa protein that is a member of the RRM family of RNA-binding proteins.  RBPMS has been identified as a critical splicing regulator in differentiated vascular smooth muscle cells.  RBPMS is expressed at high levels in the heart, breasts, lungs, kidneys, stomach, muscles, liver, eyes, adipose tissue, and ovaries.  Studies in breast cancer and ovarian cancer show that RBPMS has a potential role as a tumor suppressor.

1. Nakagaki-Silva EE, Gooding C, Llorian M, Jacob AG, Richards F, Buckroyd A, Sinha S, Smith CWJ. Identification of RBPMS as a mammalian smooth muscle master splicing regulator via proximity of its gene with super-enhancers. Elife. 2019 Jul 8;8:e46327. doi: 10.7554/eLife.46327. PMID: 31283468; PMCID: PMC6613909. 
2. Rabelo-Fernández RJ, Santiago-Sánchez GS, Sharma RK, Roche-Lima A, Carrion KC, Rivera RAN, Quiñones-Díaz BI, Rajasekaran S, Siddiqui J, Miles W, Rivera YS, Valiyeva F, Vivas-Mejia PE. Reduced RBPMS Levels Promote Cell Proliferation and Decrease Cisplatin Sensitivity in Ovarian Cancer Cells. Int J Mol Sci. 2022 Jan 4;23(1):535. doi: 10.3390/ijms23010535. PMID: 35008958; PMCID: PMC8745614.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q93062(Human)