RBP4/Retinol-Binding Protein 4 Antibody (4C2)

Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Western blot analysis using RBP4 mAb against HEK293 (1) and RBP4(AA: 1-201)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Immunofluorescence analysis of HepG2 cells using RBP4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and stomach cancer tissues (right) using RBP4 mouse mAb with DAB staining.

Flow Cytometry: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Flow cytometric analysis of HepG2 cells using RBP4 mouse mAb (green) and negative control (purple).

ELISA: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 4C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

RBP4/Retinol-Binding Protein 4 Antibody (4C2) Summary

• Immunogen: Purified recombinant fragment of human RBP expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBP4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for RBP4/Retinol-Binding Protein 4 Antibody (4C2)

• interstitial
• Plasma retinol-binding protein
• RBP4
• retinol binding protein 4, plasma
• RetinolBinding Protein 4
• retinol-binding protein 4, plasma

Background

RBP4 is a specific carrier for retinol in the blood. It is a member of the lipocalin protein family. Under physiological conditions, RBP binds transthyretin (TTR) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The RBP/TTR molar ration provides an indirect way to indicate marginal vitamin A deficiency. RBP4 may also play a role in insulin resistance in diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBP4