RBP4/Retinol-Binding Protein 4 Antibody (393004) [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA(Det)
Clone
393004
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

RBP4/Retinol-Binding Protein 4 Antibody (393004) [Biotin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human RBP4/Retinol‑Binding Protein 4
Glu19-Leu201
Accession # P02753
Specificity
Detects human RBP4/Retinol‑Binding Protein 4 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant mouse RBP4 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RBP4
Purity
Protein A or G purified from ascites
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human RBP4/Retinol-Binding Protein 4 Antibody (Catalog # MAB33781)
ELISA Detection: Human RBP4/Retinol-Binding Protein 4 Biotinylated Antibody (Catalog # BAM33782)
Standard: Recombinant Human RBP4/Retinol-Binding Protein 4 (Catalog # 3378-LC)
Readout System

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from ascites
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RBP4/Retinol-Binding Protein 4 Antibody (393004) [Biotin]

  • interstitial
  • Plasma retinol-binding protein
  • RBP4
  • retinol binding protein 4, plasma
  • RetinolBinding Protein 4
  • retinol-binding protein 4, plasma

Background

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution (1, 2). However, retinol becomes quite stable and soluble in plasma due to its tight interaction with retinol-binding protein 4 (RBP4), also known as plasma retinol-binding protein. A prototypic member of the lipocalin superfamily, RBP4 has a beta -barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision.

  1. Zanotti, G. and R. Berni. (2004) Vitamins and Hormones 69:271.
  2. Newcomer, M.E. and D.E. Ong. (2000) Biochim. Biophys. Acta 1482:57.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAM33782

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Bioinformatics

Gene Symbol RBP4
Uniprot