RBM25 Antibody - Azide and BSA Free

Western Blot: RBM25 Antibody [NBP2-93951] - Analysis of extracts of various cell lines, using RBM25 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.

Immunocytochemistry/Immunofluorescence: RBM25 Antibody [NBP2-93951] - Analysis of U-2 OS cells using RBM25 at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

RBM25 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit RBM25 Antibody - Azide and BSA Free (NBP2-93951) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 614-843 of human RBM25 (NP_067062.1). KPTLRPISSAPSVSSASGNATPNTPGDESPCGIIIPHENSPDQQQPEEHRPKIGLSLKLGASNSPGQPNSVKRKKLPVDSVFNKFEDEDSDDVPRKRKLVPLDYGEDDKNATKGTVNTEEKRKHIKSLIEKIPTAKPELFAYPLDWSIVDSILMERRIRPWINKKIIEYIGEEEATLVDFVCSKVMAHSSPQSILDDVAMVLDEEAEVFIVKMWRLLIYETEAKKIGLVK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM25
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:200-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for RBM25 Antibody - Azide and BSA Free

• Arg/Glu/Asp-rich protein of 120 kDa
• Arg/Glu/Asp-rich protein, 120 kDa
• fSAP94
• functional spliceosome-associated protein 94
• MGC105088
• MGC117168
• NET52
• Protein S164
• RED120RNA-binding region-containing protein 7
• RNA binding motif protein 25
• RNA-binding motif protein 25
• RNA-binding protein 25
• RNA-binding region (RNP1, RRM) containing 7
• RNPC7
• S164
• Snu71
• U1 small nuclear ribonucleoprotein 1SNRP homolog

Background

RBM25, also identified as hRED120 (for human Arg/Glu/Asp-rich protein of 120 kDa), contains an RNA recognition motif and a PWI nucleic acid binding domain and is suggested to have a role in RNA processing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: RBM25