SRRM1 Antibody - BSA Free

Western Blot: SRRM1 Antibody [NBP2-13381] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: NTERA-2 High

Immunocytochemistry/Immunofluorescence: SRRM1 Antibody [NBP2-13381] - Staining of human cell line HEK 293 shows localization to nuclear speckles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SRRM1 Antibody [NBP2-13381] - Staining of human colon shows strong nuclear positivity in glandular cells.

Simple Western: SRRM1 Antibody [NBP2-13381] - Simple Western lane view shows a specific band for SRRM1 in 0.2 mg/ml of HEK 293T lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: SRRM1 Antibody [NBP2-13381] - Electropherogram image of the corresponding Simple Western lane view. SRRM1 antibody was used at 1:50 dilution on HEK 293T lysate(s) respectively.

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line NTERA-2

Staining of human kidney shows moderate nuclear positivity in cells in tubules and cells in glomeruli.

Staining of human colon shows strong nuclear positivity in glandular cells.

Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SRRM1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: RQRTKSRSPSPAPEKKEKTPELPEPSVKVKEPSVQEATSTSDILKVPKPEPIPEPKEPSPE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRRM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HEK 293T lysate, separated by Size, antibody dilution of 1:50, apparent MW was 203 kDa.
• Control Peptide:

  SRRM1 Protein (NBP2-13381PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SRRM1 Antibody - BSA Free

• MGC39488
• POP101
• Ser/Arg-related nuclear matrix protein
• serine/arginine repetitive matrix 1
• serine/arginine repetitive matrix protein 1
• SRm160
• SRM160160-KD
• SR-related nuclear matrix protein of 160 kDa

Background

Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRRM1 Antibody (NBP2-13381)(2)

Publications using NBP2-13381

• Tiboldi A, Fuhrer J, Schaubmayr W et al. Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells Antioxidants (Basel, Switzerland) 2021-12-04 [PMID: 34943050]
• Rossi F, Beltran M, Damizia M et al. Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity Molecular cell 2022-01-06 [PMID: 34942120]

Bioinformatics

• Gene Symbol: SRRM1