SRRM1 Antibody - BSA Free

Simple Western: SRRM1 Antibody [NBP2-13381] - Simple Western lane view shows a specific band for SRRM1 in 0.2 mg/ml of HEK 293T lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: SRRM1 Antibody [NBP2-13381] - Electropherogram image of the corresponding Simple Western lane view. SRRM1 antibody was used at 1:50 dilution on HEK 293T lysate(s) respectively.

Analysis in human cell line NB4.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SRRM1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SRRM1 Antibody - BSA Free (NBP2-13381) is a polyclonal antibody validated for use in WB and Simple Western. Anti-SRRM1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to the amino acids: RQRTKSRSPSPAPEKKEKTPELPEPSVKVKEPSVQEATSTSDILKVPKPEPIPEPKEPSPE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRRM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in HEK 293T lysate, separated by Size, antibody dilution of 1:50, apparent MW was 203 kDa.
• Control Peptide:

  SRRM1 Protein (NBP2-13381PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SRRM1 Antibody - BSA Free

• MGC39488
• POP101
• Ser/Arg-related nuclear matrix protein
• serine/arginine repetitive matrix 1
• serine/arginine repetitive matrix protein 1
• SRm160
• SRM160160-KD
• SR-related nuclear matrix protein of 160 kDa

Background

Part of pre- and post-splicing multiprotein mRNP complexes. Involved in numerous pre-mRNA processing events. Promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. Stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. Binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. Binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRRM1 Antibody (NBP2-13381)(2)

Publications using NBP2-13381

• Tiboldi A, Fuhrer J, Schaubmayr W et al. Oxygen-Dependent Changes in the N-Glycome of Murine Pulmonary Endothelial Cells Antioxidants (Basel, Switzerland) 2021-12-04 [PMID: 34943050]
• Rossi F, Beltran M, Damizia M et al. Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity Molecular cell 2022-01-06 [PMID: 34942120]

Bioinformatics

• Gene Symbol: SRRM1