RBFOX3/NeuN Antibody (3202C) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clone: 3202C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 555

RBFOX3/NeuN Antibody (3202C) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects a synthetic peptide specific for human RBFOX3 around amino acid 25 in Direct ELISA.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for RBFOX3/NeuN Antibody (3202C) [Alexa Fluor™ Plus 555]

• FLJ56884
• FLJ58356
• Fox-1 homolog C
• FOX3
• FOX-3
• hexaribonucleotide binding protein 3
• HRNBP3
• neuN antigen
• NEUN
• neuronal nuclei antigen
• neuronal nuclei
• RBFOX3
• RNA binding protein fox-1 homolog 3
• RNA binding protein, fox-1 homolog (C. elegans) 3
• RNA binding protein, fox-1 homolog 3

Background

RBFOX3, also known as Neuronal nuclei (NeuN), is a 34kDa marker of post-mitotic neurons that is highly conserved  among different species.  The RBFOX family regulates alternative slicing and is encoded by three genes: RBFOX1, RBFOX2 and RBFOX3.  RBFOX3 mediates hippocampal circuitry, neurogenesis, and synaptogenesis. RBFOX3 plays a crucial role in normal synaptic function and is implicated in human neurological functions and mutations have been linked to neurodevelopmental delay, cognitive impairment, autistic features, and epilepsy.

1. Wang HY, Hsieh PF, Huang DF, Chin PS, Chou CH, Tung CC, Chen SY, Lee LJ, Gau SS, Huang HS. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep. 2015 Dec 1;5:17383. doi: 10.1038/srep17383. PMID: 26619789; PMCID: PMC4664964. 
2. Huang DF, Lee CY, Chou MY, Yang TY, Cao X, Hsiao YH, Wu RN, Lien CC, Huang YS, Huang HP, Gau SS, Huang HS. Neuronal splicing regulator RBFOX3 mediates seizures via regulating Vamp1 expression preferentially in NPY-expressing GABAergic neurons. Proc Natl Acad Sci U S A. 2022 Aug 16;119(33):e2203632119. doi: 10.1073/pnas.2203632119. Epub 2022 Aug 11. PMID: 35951651; PMCID: PMC9388145.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.