RB1 [p Ser795] Antibody

Western Blot: RB1 [p Ser795] Antibody [NB100-82176] - Analysis of RB1 [p Ser795] ( using NB100-82176 in K562 cells untreated or treated with 10% serum after 48 hours of starvation.

Immunocytochemistry/Immunofluorescence: RB1 [p Ser795] Antibody [NB100-82176] - Staining of methanol-fixed Hela cells using Rb1 (P Ser795) antibody

Immunohistochemistry: RB1 [p Ser795] Antibody [NB100-82176] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Rb1 (P Ser795) antibody.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

RB1 [p Ser795] Antibody Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human RB1 around the phosphorylation site of serine 795 (P-S-SP-P-L).
• Modification: p Ser795
• Specificity: Detects endogenous levels of RB1 only when phosphorylated at serine 795.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RB1 [p Ser795] Antibody

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RB1
• Entrez:
  • 5925(Human)
  • 19645(Mouse)
  • 24708(Rat)
• Uniprot:
  • P06400()