RB1 Antibody

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Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, EqSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RB1 Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 251-268 (RTPRRGQNRSARIAKQLE) of human Rb was used as immunogen
Specificity
In Jurkat, a 110 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RB1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:1000
Positive Control
Jurkat Lysate (NB800-PC2)
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-56888 in the following application:

Reactivity Notes

Cross reacts with Dog, Horse, Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for RB1 Antibody

  • OSRC
  • osteosarcoma
  • p105-Rb
  • pp110
  • pRb
  • RB
  • RB1
  • retinoblastoma 1
  • retinoblastoma suspectibility protein
  • retinoblastoma-associated protein

Background

Mutation or deregulation of Rb, retinoblastoma tumor suppressor gene, is seen so frequently in tumor cells that it is believe that some type of suppression of the Rb gene is required for cancer cell genesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for RB1 Antibody (NB100-56888) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NB100-56888:
Filter by Applications
WB
(2)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot RB1 NB100-56888
Enlarge
reviewed by:
Anonymous
WB Human 07/31/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell extract form Jurkat cells
SpeciesHuman
CommentsAs all other antibodies this one also give multiple bands but a prominent band is visible at the right size.

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:1000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 30 sec

Comments

CommentsAs all other antibodies this one also give multiple bands but a prominent band is visible at the right size.
Western Blot RB1 NB100-56888
Enlarge
reviewed by:
Anonymous
WB Human 07/25/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell extract from 293T cells
SpeciesHuman
CommentsThe antibody gives a little bit of background as with all other RB antibodies

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP (SCBT)
Secondary Concentration1:20,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Comments

CommentsThe antibody gives a little bit of background as with all other RB antibodies

Product General Protocols

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Video Protocols

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FAQs for RB1 Antibody (NB100-56888) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for RB1 Antibody (NB100-56888)

Discover related pathways, diseases and genes to RB1 Antibody (NB100-56888). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for RB1 Antibody (NB100-56888)

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Research Areas for RB1 Antibody (NB100-56888)

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Blogs on RB1

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Recent Reviews

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4
2
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0
2
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1
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Anonymous
07/31/2014
Application: WB
Species: Human

Anonymous
07/25/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RB1
Entrez
Uniprot