Rat IL-4 Quantikine ELISA Kit

• Reactivity: Rt
• Applications: ELISA
• Conjugate: HRP

Rat IL-4 Quantikine ELISA Kit Summary

• Background: The Quantikine Rat IL-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-4 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant rat IL-4 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-4 accurately. Results obtained using natural rat IL-4 showed dose res...ponse curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-4.
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• Specificity: Natural and recombinant rat IL-4
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IL-4 Quantikine ELISA Kit

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-4 (R4000)(15)

Publications using R4000

• Zhu, WX;Xi, Y;Li, F;Jiao, WE;Li, ZJ;Chen, SM;Kong, YG;Xu, Y;Deng, YQ;Zuo, JJ;Tao, ZZ; Calpeptin may reverse glucocorticoid-resistance of allergic rhinitis associated with cigarette smoke exposure by down-regulating interferon regulatory factor 1 Heliyon 2023-07-01 [PMID: 37449098] (Rat)
• YX Ma, K Jiao, QQ Wan, J Li, MY Liu, ZB Zhang, W Qin, KY Wang, YZ Wang, FR Tay, LN Niu Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration Bioactive materials, 2021-07-27;9(0):475-490. 2021-07-27 [PMID: 34820584] (Rat)
• RVP Neves, HL Corrêa, IV de Sousa N, MK Souza, F Costa, AS Haro, LA Deus, AL Reis, HG Simões, RV Andrade, CO Assumpção, W Stone, J Prestes, EC Vieira, R de Cássia, V Cruzat, TS Rosa Renoprotection Induced by Aerobic Training Is Dependent on Nitric Oxide Bioavailability in Obese Zucker Rats Oxidative Medicine and Cellular Longevity, 2021-09-28;2021(0):3683796. 2021-09-28 [PMID: 34621463] (Rat)
• JD Koerner, DZ Markova, GD Schroeder, BP Calio, A Shah, CW Brooks, AR Vaccaro, DG Anderson, CK Kepler The local cytokine and growth factor response to rhBMP-2 after spinal fusion Spine J, 2018-03-14;0(0):. 2018-03-14 [PMID: 29550606] (ELISA (Standard), Rat)
• B Nieto-Lima, A Cano-Martí, ME Rubio-Ruiz, I Pérez-Torr, V Guarner-La Age-, Gender-, andin VivoDifferent Doses of Isoproterenol Modifyin VitroAortic Vasoreactivity and Circulating VCAM-1 Front Physiol, 2018-01-24;9(0):20. 2018-01-24 [PMID: 29416512] (Mouse)
• BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus) PLoS ONE, 2017-12-13;12(12):e0188779. 2017-12-13 [PMID: 29236720] (Rat)
• C Honjoh, K Chihara, H Yoshiki, S Yamauchi, K Takeuchi, Y Kato, Y Hida, T Ishizuka, K Sada Association of C-Type Lectin Mincle with Fc?RI?? Subunits Leads to Functional Activation of RBL-2H3 Cells through Syk Sci Rep, 2017-04-10;7(0):46064. 2017-04-10 [PMID: 28393919] (Rat)
• Gao L, Lu Q, Huang L, Ruan L, Yang J, Huang W, ZhuGe W, Zhang Y, Fu B, Jin K, ZhuGe Q Transplanted neural stem cells modulate regulatory T, gammadelta T cells and corresponding cytokines after intracerebral hemorrhage in rats. Int J Mol Sci, 2014-03-13;15(3):4431-41. 2014-03-13 [PMID: 24633197] (Rat)
• Joo H, Nam S, Yang K, Kim C, Jin Y, Kim J The effects of low-dose ionizing radiation in the activated rat basophilic leukemia (RBL-2H3) mast cells. J Biol Chem, 2012-06-14;287(33):27789-95. 2012-06-14 [PMID: 22700973] (Rat)
• Stevenson CS, Docx C, Webster R, Battram C, Hynx D, Giddings J, Cooper PR, Chakravarty P, Rahman I, Marwick JA, Kirkham PA, Charman C, Richardson DL, Nirmala NR, Whittaker P, Butler K Comprehensive gene expression profiling of rat lung reveals distinct acute and chronic responses to cigarette smoke inhalation. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-08-24;293(5):L1183-93. 2007-08-24 [PMID: 17720875] (Rat)
• de Oliveira AP, Domingos HV, Cavriani G, Damazo AS, Dos Santos Franco AL, Oliani SM, Oliveira-Filho RM, Vargaftig BB, de Lima WT Cellular recruitment and cytokine generation in a rat model of allergic lung inflammation are differentially modulated by progesterone and estradiol. Am. J. Physiol., Cell Physiol., 2007-07-18;293(3):C1120-8. 2007-07-18 [PMID: 17634417] (Rat)
• Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction. J. Immunol., 2005-02-15;174(4):2327-35. 2005-02-15 [PMID: 15699169] (Rat)
• Schubert T, Horch RE Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma. Ann. Surg., 2004-08-01;240(2):340-9. 2004-08-01 [PMID: 15273560] (Rat)
• Jeschke MG, Klein D, Bolder U, Einspanier R Insulin attenuates the systemic inflammatory response in endotoxemic rats. Endocrinology, 2004-06-10;145(9):4084-93. 2004-06-10 [PMID: 15192048] (Rat)
• de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans. Infect. Immun., 2000-06-01;68(6):3297-304. 2000-06-01 [PMID: 10816477] (Rat)

FAQs for IL-4 (R4000). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: Il4