Rat IL-2 Quantikine ELISA Kit

• Reactivity: Rt
• Applications: ELISA
• Conjugate: HRP

Rat IL-2 Quantikine ELISA Kit Summary

• Background: The Quantikine Rat IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural rat IL-2 showed d...ose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-2.
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• Specificity: Natural and recombinant rat IL-2
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IL-2 Quantikine ELISA Kit

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-2 (R2000)(21)

Publications using R2000

• Kirkland, JM;Patel, I;Ardeshna, MS;Kopec, AM; Microglial synaptic pruning in the nucleus accumbens during adolescence sex-specifically influences splenic immune outcomes bioRxiv : the preprint server for biology 2023-05-05 [PMID: 37205376] (Rat)
• YX Ma, K Jiao, QQ Wan, J Li, MY Liu, ZB Zhang, W Qin, KY Wang, YZ Wang, FR Tay, LN Niu Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration Bioactive materials, 2021-07-27;9(0):475-490. 2021-07-27 [PMID: 34820584] (Rat)
• I Belinchón-, P Bellot, D Romero-Pér, I Herraiz-Ro, F Marco, R Frances, JM Ramos-Rinc Non-alcoholic fatty liver disease is associated with bacterial translocation and a higher inflammation response in psoriatic patients Scientific Reports, 2021-04-21;11(1):8593. 2021-04-21 [PMID: 33883616] (Rat)
• B Xiao, Y Zhang, S Liu, J Ding, Z Yu, T Wang, B Song Nuclear Receptor Subfamily 4 Group A Member 1 Overexpression Prolongs Free Flap Allotransplant Graft Survival by Inducing T-Cell Anergy in the Rat Transplant. Proc., 2020-09-09;0(0):. 2020-09-09 [PMID: 32919804] (Rat)
• IS Dhande, SC Kneedler, Y Zhu, AS Joshi, MJ Hicks, SE Wenderfer, MC Braun, PA Doris Natural genetic variation in Stim1 creates stroke in the spontaneously hypertensive rat Genes Immun., 2020-04-17;0(0):. 2020-04-17 [PMID: 32300198] (Rat)
• Liu C, Hong T, Shao M, Chen Z, Wang C Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis. Mol Med Rep, 2014-07-15;10(3):1323-8. 2014-07-15 [PMID: 25059226] (Rat)
• Wojcikowski J, Golembiowska K, Daniel WA Regulation of liver cytochrome P450 by activation of brain dopaminergic system: physiological and pharmacological implications. Biochem. Pharmacol., 2008-05-03;76(2):258-67. 2008-05-03 [PMID: 18538745] (Rat)
• Foulds LM, Boysen RI, Crane M, Yang Y, Muir JA, Smith AI, de Kretser DM, Hearn MT, Hedger MP Molecular identification of lyso-glycerophosphocholines as endogenous immunosuppressives in bovine and rat gonadal fluids. Biol. Reprod., 2008-05-28;79(3):525-36. 2008-05-28 [PMID: 18509166] (Rat)
• Hu AP, Du JM, Li JY, Liu JW Oridonin promotes CD4+/CD25+ Treg differentiation, modulates Th1/Th2 balance and induces HO-1 in rat splenic lymphocytes. Inflamm. Res., 2008-04-01;57(4):163-70. 2008-04-01 [PMID: 18351433] (Rat)
• Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction. J. Immunol., 2005-02-15;174(4):2327-35. 2005-02-15 [PMID: 15699169] (Rat)
• Labuzek K, Kowalski J, Gabryel B, Herman ZS Chlorpromazine and loxapine reduce interleukin-1beta and interleukin-2 release by rat mixed glial and microglial cell cultures. Eur Neuropsychopharmacol, 2005-01-01;15(1):23-30. 2005-01-01 [PMID: 15572270] (Rat)
• Schubert T, Horch RE Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma. Ann. Surg., 2004-08-01;240(2):340-9. 2004-08-01 [PMID: 15273560] (Rat)
• Jeschke MG, Klein D, Bolder U, Einspanier R Insulin attenuates the systemic inflammatory response in endotoxemic rats. Endocrinology, 2004-06-10;145(9):4084-93. 2004-06-10 [PMID: 15192048] (Rat)
• Tagawa T, Kozower BD, Kanaan SA, Daddi N, Muraoka M, Oka T, Ritter JH, Patterson GA Gene transfer of tumor necrosis factor inhibitor improves the function of lung allografts. J. Thorac. Cardiovasc. Surg., 2004-06-01;127(6):1558-63. 2004-06-01 [PMID: 15173707] (Rat)
• Tryphonas H, Cooke G, Caldwell D, Bondy G, Parenteau M, Hayward S, Pulido O Oral (gavage), in utero and post-natal exposure of Sprague-Dawley rats to low doses of tributyltin chloride. Part II: effects on the immune system. Food Chem. Toxicol., 2004-02-01;42(2):221-35. 2004-02-01 [PMID: 14667469] (Rat)
• Zhao W, Kobayashi M, Ding W, Yuan L, Seth P, Cornain S, Wang J, Okada F, Hosokawa M Suppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-beta receptor type II. Cancer Immunol. Immunother., 2002-06-25;51(7):381-8. 2002-06-25 [PMID: 12192538] (Rat)
• D'Ovidio F Recipient intramuscular cotransfection of naked plasmid transforming growth factor beta1 and interleukin 10 ameliorates lung graft ischemia-reperfusion injury. J. Thorac. Cardiovasc. Surg., 2002-08-01;124(2):259-69. 2002-08-01 [PMID: 12167785] (Rat)
• Jimenez E, Sacedon R, Vicente A, Hernandez-Lopez C, Zapata AG, Varas A Rat peripheral CD4+CD8+ T lymphocytes are partially immunocompetent thymus-derived cells that undergo post-thymic maturation to become functionally mature CD4+ T lymphocytes. J. Immunol., 2002-05-15;168(10):5005-13. 2002-05-15 [PMID: 11994452] (Rat)
• Pahlavani MA, Vargas DA, Evans TR, Shu JH, Nelson JF Melatonin fails to modulate immune parameters influenced by calorie restriction in aging Fischer 344 rats. Exp. Biol. Med. (Maywood), 2002-03-01;227(3):201-7. 2002-03-01 [PMID: 11856819] (Rat)
• de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans. Infect. Immun., 2000-06-01;68(6):3297-304. 2000-06-01 [PMID: 10816477] (Rat)
• Kuiper M, Sanches R, Gaken JA, Bignon YJ Cloning and characterization of a retroviral plasmid, pCC1, for combination suicide gene therapy. BioTechniques, 2000-03-01;28(3):572-4, 576. 2000-03-01 [PMID: 10723573] (Rat)

Bioinformatics

• Gene Symbol: Il2