Rat IFN-gamma Quantikine ELISA Kit

• Reactivity: Rt
• Applications: ELISA
• Conjugate: HRP

Rat IFN-gamma Quantikine ELISA Kit Summary

• Background: The Quantikine Rat IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IFN-gamma levels in cell culture supernates and serum. It contains E. coli-expressed recombinant rat IFN-gamma and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant rat IFN-gamma accurately. Results obtained using natural rat IFN-g...amma showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IFN-gamma.
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• Specificity: Natural and recombinant rat IFN-gamma
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ifng

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IFN-gamma Quantikine ELISA Kit

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IFN-gamma (RIF00)(32)

Publications using RIF00

• Kirkland, JM;Patel, I;Ardeshna, MS;Kopec, AM; Microglial synaptic pruning in the nucleus accumbens during adolescence sex-specifically influences splenic immune outcomes bioRxiv : the preprint server for biology 2023-05-05 [PMID: 37205376] (Rat)
• YX Ma, K Jiao, QQ Wan, J Li, MY Liu, ZB Zhang, W Qin, KY Wang, YZ Wang, FR Tay, LN Niu Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration Bioactive materials, 2021-07-27;9(0):475-490. 2021-07-27 [PMID: 34820584] (Rat)
• L Zheng, R Tang, L Shi, M Zhong, Z Zhou Vagus nerve stimulation ameliorates L-NAME-induced preeclampsia-like symptoms in rats through inhibition of the inflammatory response BMC pregnancy and childbirth, 2021-03-04;21(1):177. 2021-03-04 [PMID: 33663436] (Rat)
• F Yaghouby, K Jang, U Hoang, S Asgari, S Vasudevan Sex Differences in Vagus Nerve Stimulation Effects on Rat Cardiovascular and Immune Systems Front Neurosci, 2020-11-06;14(0):560668. 2020-11-06 [PMID: 33240036] (Rat)
• B Xiao, Y Zhang, S Liu, J Ding, Z Yu, T Wang, B Song Nuclear Receptor Subfamily 4 Group A Member 1 Overexpression Prolongs Free Flap Allotransplant Graft Survival by Inducing T-Cell Anergy in the Rat Transplant. Proc., 2020-09-09;0(0):. 2020-09-09 [PMID: 32919804] (Rat)
• S Sekar, SK Panchal, NK Ghattamane, L Brown, R Crawford, Y Xiao, I Prasadam Dietary Saturated Fatty Acids Modulate Pain Behaviour in Trauma-Induced Osteoarthritis in Rats Nutrients, 2020-02-18;12(2):. 2020-02-18 [PMID: 32085385] (Rat)
• E Ramírez, C Sánchez-Ma, MA Mayoral, L Mendieta, V Alatriste, A Patricio-M, ID Limón Neuroinflammation induced by the peptide amyloid-? (25-35) increase the presence of galectin-3 in astrocytes and microglia and impairs spatial memory Neuropeptides, 2019-02-14;0(0):. 2019-02-14 [PMID: 30795916] (Rat)
• BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus) PLoS ONE, 2017-12-13;12(12):e0188779. 2017-12-13 [PMID: 29236720] (Rat)
• JW Jeong, HH Lee, J Kim, EO Choi, H Hwang-Bo, HJ Kim, MY Kim, KI Ahn, GY Kim, KW Lee, KY Kim, SG Kim, SH Hong, C Park, HJ Cha, YH Choi Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate?induced osteoarthritis rats Mol Med Rep, 2017-07-21;0(0):. 2017-07-21 [PMID: 29067461] (Rat)
• WP Zheng, BY Zhang, ZY Shen, ML Yin, Y Cao, HL Song Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in�vitro Mol Med Rep, 2017-03-15;15(5):2551-2559. 2017-03-15 [PMID: 28447750] (Rat)
• EY Chiang, T Li, S Jeet, I Peng, J Zhang, WP Lee, J DeVoss, P Caplazi, J Chen, S Warming, DH Hackos, S Mukund, CM Koth, JL Grogan Potassium channels Kv1.3 and KCa3.1 cooperatively and compensatorily regulate antigen-specific memory T cell functions Nat Commun, 2017-03-01;8(0):14644. 2017-03-01 [PMID: 28248292] (Rat)
• ME El-Housein, IA El-Agoza, MM Sakr, GM El-Malky Novel protective role of curcumin and taurine combination against experimental hepatocarcinogenesis Exp Ther Med, 2016-12-02;13(1):29-36. 2016-12-02 [PMID: 28123463] (Rat)
• Garrido P, Ribeiro S, Fernandes J, Vala H, Bronze-da-Rocha E, Rocha-Pereira P, Belo L, Costa E, Santos-Silva A, Reis F Iron-hepcidin dysmetabolism, anemia and renal hypoxia, inflammation and fibrosis in the remnant kidney rat model. PLoS ONE, 2015-04-13;10(4):e0124048. 2015-04-13 [PMID: 25867633] (Rat)
• Chuffa L, Fioruci-Fontanelli B, Mendes L, Ferreira Seiva F, Martinez M, Favaro W, Domeniconi R, Pinheiro P, Delazari Dos Santos L, Martinez F Melatonin attenuates the TLR4-mediated inflammatory response through MyD88- and TRIF-dependent signaling pathways in an in vivo model of ovarian cancer. BMC Cancer, 2015-02-06;15(0):34. 2015-02-06 [PMID: 25655081] (Rat)
• Gao L, Lu Q, Huang L, Ruan L, Yang J, Huang W, ZhuGe W, Zhang Y, Fu B, Jin K, ZhuGe Q Transplanted neural stem cells modulate regulatory T, gammadelta T cells and corresponding cytokines after intracerebral hemorrhage in rats. Int J Mol Sci, 2014-03-13;15(3):4431-41. 2014-03-13 [PMID: 24633197] (Rat)
• Hu AP, Du JM, Li JY, Liu JW Oridonin promotes CD4+/CD25+ Treg differentiation, modulates Th1/Th2 balance and induces HO-1 in rat splenic lymphocytes. Inflamm. Res., 2008-04-01;57(4):163-70. 2008-04-01 [PMID: 18351433] (Rat)
• Stevenson CS, Docx C, Webster R, Battram C, Hynx D, Giddings J, Cooper PR, Chakravarty P, Rahman I, Marwick JA, Kirkham PA, Charman C, Richardson DL, Nirmala NR, Whittaker P, Butler K Comprehensive gene expression profiling of rat lung reveals distinct acute and chronic responses to cigarette smoke inhalation. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-08-24;293(5):L1183-93. 2007-08-24 [PMID: 17720875] (Rat)
• Sheikh N, Dudas J, Ramadori G Changes of gene expression of iron regulatory proteins during turpentine oil-induced acute-phase response in the rat. Lab. Invest., 2007-04-09;87(7):713-25. 2007-04-09 [PMID: 17417667] (Rat)
• Griffin R, Nally R, Nolan Y, McCartney Y, Linden J, Lynch MA The age-related attenuation in long-term potentiation is associated with microglial activation. J. Neurochem., 2006-09-18;99(4):1263-72. 2006-09-18 [PMID: 16981890] (Rat)
• Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat. Crit. Care Med., 2005-10-01;33(10):2317-22. 2005-10-01 [PMID: 16215387] (Rat)
• Pandha HS, Cook D, Greenhalgh R, Dalgleish A Immunotherapy of murine prostate cancer using whole tumour cells killed ex vivo by cytosine deaminase/5-fluorocytosine suicide-gene therapy. BJU Int., 2005-06-01;95(9):1336-43. 2005-06-01 [PMID: 15892827] (Rat)
• Fang IM, Yang CH, Lin CP, Yang CM, Chen MS Effects of pyrrolidine dithiocarbamate, an NF-kappaB inhibitor, on cytokine expression and ocular inflammation in experimental autoimmune anterior uveitis. J Ocul Pharmacol Ther, 2005-04-01;21(2):95-106. 2005-04-01 [PMID: 15857275] (Rat)
• Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction. J. Immunol., 2005-02-15;174(4):2327-35. 2005-02-15 [PMID: 15699169] (Rat)
• Stasi MA, Ruggiero V, Ursillo A, Taurelli R, Agliano M, Weber E, Lorenzoni P, Sorrentino V, Pacifici L, Carminati P Ameliorating effects of the immunomodulator 3-(2-ethylphenyl)-5-(3-methoxyphenyl)-1H-1,2,4-triazole in an experimental model of colitis in the rat. Eur. J. Pharmacol., 2004-06-28;494(2):263-72. 2004-06-28 [PMID: 15212983] (Rat)
• Tucker SN, Lin K, Stevens S, Scollay R, Bennett MJ, Olson DC Salivary gland genetic vaccination: a scalable technology for promoting distal mucosal immunity and heightened systemic immune responses. Vaccine, 2004-06-23;22(19):2500-4. 2004-06-23 [PMID: 15193415] (Rat)
• Tagawa T, Kozower BD, Kanaan SA, Daddi N, Muraoka M, Oka T, Ritter JH, Patterson GA Gene transfer of tumor necrosis factor inhibitor improves the function of lung allografts. J. Thorac. Cardiovasc. Surg., 2004-06-01;127(6):1558-63. 2004-06-01 [PMID: 15173707] (Rat)
• Yuan Z, Liu Y, Liu Y, Zhang J, Kishimoto C, Ma A, Liu Z Peroxisome proliferator-activated receptor-gamma ligands ameliorate experimental autoimmune myocarditis associated with inhibition of self-sensitive T cells. J. Cardiovasc. Pharmacol., 2004-06-01;43(6):868-75. 2004-06-01 [PMID: 15167281] (Rat)
• Morgan DL, Shines CJ Alveolar macrophage cytotoxicity for normal lung fibroblasts is mediated by nitric oxide release. Toxicol In Vitro, 2004-02-01;18(1):139-46. 2004-02-01 [PMID: 14630072] (Rat)
• D'Ovidio F Recipient intramuscular cotransfection of naked plasmid transforming growth factor beta1 and interleukin 10 ameliorates lung graft ischemia-reperfusion injury. J. Thorac. Cardiovasc. Surg., 2002-08-01;124(2):259-69. 2002-08-01 [PMID: 12167785] (Rat)
• Pahlavani MA, Vargas DA, Evans TR, Shu JH, Nelson JF Melatonin fails to modulate immune parameters influenced by calorie restriction in aging Fischer 344 rats. Exp. Biol. Med. (Maywood), 2002-03-01;227(3):201-7. 2002-03-01 [PMID: 11856819] (Rat)
• Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA Role of IL-18 in acute lung inflammation. J. Immunol., 2001-12-15;167(12):7060-8. 2001-12-15 [PMID: 11739527] (Rat)
• Ribault S, Neuville P, Mechine-Neuville A, Auge F, Parlakian A, Gabbiani G, Paulin D, Calenda V Chimeric smooth muscle-specific enhancer/promoters: valuable tools for adenovirus-mediated cardiovascular gene therapy. Circ. Res., 2001-03-16;88(5):468-75. 2001-03-16 [PMID: 11249869] (Rat)

FAQs for IFN-gamma (RIF00). (Showing 1 - 1 of 1 FAQs).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: Ifng