Rat GM-CSF DuoSet ELISA, 15 Plate

• Reactivity: Rt
• Applications: ELISA
• Conjugate: Biotin

Rat GM-CSF DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Csf2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat GM-CSF DuoSet ELISA, 15 Plate

• colony stimulating factor 2 (granulocyte-macrophage)
• Colony-stimulating factor
• CSF
• CSF2
• CSF-2
• GMCSF
• GM-CSF
• GMCSFgranulocyte-macrophage colony-stimulating factor
• granulocyte-macrophage colony stimulating factor
• MGC131935
• MGC138897
• Molgramostim
• molgramostin
• Sargramostim

Background

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.

Publications for GM-CSF (DY518)(5)

Publications using DY518

• MJ Jeong, S Jeon, HS Yu, WS Cho, S Lee, D Kang, Y Kim, YJ Kim, SY Kim Exposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome International Journal of Environmental Research and Public Health, 2022-01-04;19(1):. 2022-01-04 [PMID: 35010784] (Rat)
• R Wang, J Liu, Z Wang, X Wu, H Guo, X Jiao, H Zhang, C Qi, X Li Mangiferin exert protective effects on joints of adjuvant-induced arthritis rats by regulating the MAPKs/NF-kappaB pathway of fibroblast-like synoviocytes International immunopharmacology, 2021-11-24;0(0):108352. 2021-11-24 [PMID: 34836794] (Rat)
• Lauzon-Joset J, Marsolais D, Langlois A, Bissonnette E Dysregulation of alveolar macrophages unleashes dendritic cell-mediated mechanisms of allergic airway inflammation. Mucosal Immunol, 2013-05-29;7(1):155-64. 2013-05-29 [PMID: 23715174] (Rat)
• Reed JA, Clegg DJ, Smith KB, Tolod-Richer EG, Matter EK, Picard LS, Seeley RJ GM-CSF action in the CNS decreases food intake and body weight. J. Clin. Invest., 2005-11-01;115(11):3035-44. 2005-11-01 [PMID: 16276414] (Rat)
• Davidson MT, Deitch EA, Lu Q, Osband A, Feketeova E, Nemeth ZH, Hasko G, Xu DZ A study of the biologic activity of trauma-hemorrhagic shock mesenteric lymph over time and the relative role of cytokines. Surgery, 2004-07-01;136(1):32-41. 2004-07-01 [PMID: 15232537] (Rat)

FAQs for GM-CSF (DY518). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Csf2