Rat beta-NGF DuoSet ELISA, 15 Plate

• Reactivity: Rt
• Applications: ELISA
• Conjugate: Biotin

Rat beta-NGF DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Ngf

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat beta-NGF DuoSet ELISA, 15 Plate

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

Nerve growth factor (NGF) was initially isolated in the mouse submandibular gland over three decades ago as a 7S complex composed of three non- covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases, while the beta subunit, called beta-NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. The human protein shares approximately 90% identity at the amino acid level with both mouse and rat beta-NGF.

Publications for beta-NGF (DY556)(29)

Publications using DY556

• Zaninelli, TH;Fattori, V;Heintz, OK;Wright, KR;Bennallack, PR;Sim, D;Bukhari, H;Terry, KL;Vitonis, AF;Missmer, SA;Andrello, AC;Anchan, RM;Godin, SK;Bree, D;Verri, WA;Rogers, MS; Targeting NGF but not VEGFR1 or BDNF signaling reduces endometriosis-associated pain in mice Journal of advanced research 2024-08-12 [PMID: 39142441] (Human, Mouse, Transgenic Mouse)
• N Hamadi, ÖG Deniz, ASA Issa, AUS Islam, N Amir, ST Minhas, N Madjid, F Khelifi-To, S Kaplan, A Adem Stereological Evidence of Non-Selective Hippocampal Neurodegeneration, IGF-1 Depletion, and Behavioral Deficit following Short Term Bilateral Adrenalectomy in Wistar Rats Biomolecules, 2022-12-22;13(1):. 2022-12-22 [PMID: 36671407] (Rat)
• HJ Qin, H Li, JZ Chen, KR Zhang, XQ Zhao, JQ Qin, B Yu, J Yang Artificial nerve graft constructed by coculture of activated Schwann cells and human hair keratin for repair of peripheral nerve defects Neural regeneration research, 2023-05-01;18(5):1118-1123. 2023-05-01 [PMID: 36255001] (Rat)
• S Klein, A Siegmund, A Eigenberge, V Hartmann, F Langewost, N Hammer, A Anker, K Klein, C Morsczeck, L Prantl, O Felthaus Peripheral Nerve Regeneration-Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion Cells, 2022-09-15;11(18):. 2022-09-15 [PMID: 36139462] (Rat)
• MR Casanova, RL Reis, A Martins, NM Neves Stimulation of Neurite Outgrowth Using Autologous NGF Bound at the Surface of a Fibrous Substrate Biomolecules, 2021-12-24;12(1):. 2021-12-24 [PMID: 35053173] (Rat)
• Y Yokozeki, K Uchida, A Kawakubo, M Nakawaki, T Okubo, M Miyagi, G Inoue, M Itakura, H Sekiguchi, M Takaso TGF-beta regulates nerve growth factor expression in a mouse intervertebral disc injury model Bmc Musculoskeletal Disorders, 2021-07-23;22(1):634. 2021-07-23 [PMID: 34301215] (Mouse)
• N Sultan, LE Amin, AR Zaher, ME Grawish, BA Scheven Dental pulp stem cells stimulate neuronal differentiation of PC12 cells Neural regeneration research, 2021-09-01;16(9):1821-1828. 2021-09-01 [PMID: 33510089] (Rat)
• GR Chai, S Liu, HW Yang, XL Chen Quercetin protects against diabetic retinopathy in rats by inducing heme oxygenase-1 expression Neural regeneration research, 2021-07-01;16(7):1344-1350. 2021-07-01 [PMID: 33318415] (Rat)
• K Xi, Y Gu, J Tang, H Chen, Y Xu, L Wu, F Cai, L Deng, H Yang, Q Shi, W Cui, L Chen Microenvironment-responsive immunoregulatory electrospun fibers for promoting nerve function recovery Nat Commun, 2020-09-09;11(1):4504. 2020-09-09 [PMID: 32908131] (Rat)
• P Bortoluzzi, D Casagrande, N Dos Santos, K Ugioni Sim, C Mendes, ME Anastácio, PC Lock Silve, A Pastoris M Insulin treatment protects the brain against neuroinflammation by reducing cerebral cytokines and modulating mitochondrial function Brain Res. Bull., 2019-04-16;0(0):. 2019-04-16 [PMID: 31002914] (Rat)
• N Nagura, T Kenmoku, K Uchida, M Nakawaki, G Inoue, M Takaso Nerve growth factor continuously elevates in a rat rotator cuff tear model J Shoulder Elbow Surg, 2018-08-23;28(1):143-148. 2018-08-23 [PMID: 30146165] (Rat)
• AM Birch, ÁM Kelly Lifelong environmental enrichment in the absence of exercise protects the brain from age-related cognitive decline Neuropharmacology, 2018-04-07;0(0):. 2018-04-07 [PMID: 29630903] (Rat)
• LN Hu, JX Tian, W Gao, J Zhu, FF Mou, XC Ye, YP Liu, PP Lu, SJ Shao, HD Guo Electroacupuncture and moxibustion promote regeneration of injured sciatic nerve through Schwann cell proliferation and nerve growth factor secretion Neural Regen Res, 2018-03-01;13(3):477-483. 2018-03-01 [PMID: 29623933] (Rat)
• LN Spezia Ada, R Vercelino, C de Oliveir, VL Scarabelot, A de Souza, LF Medeiros, SG Cioato, W Caumo, ILS Torres Isoflurane and the analgesic effect of acupuncture and electroacupuncture in an animal model of neuropathic pain J Acupunct Meridian Stud, 2018-02-06;0(0):. 2018-02-06 [PMID: 29436370] (Rat)
• V Chapman, H Markides, DR Sagar, L Xu, JJ Burston, P Mapp, A Kay, RH Morris, O Kehoe, AJ El Haj Therapeutic Benefit for Late, but Not Early, Passage Mesenchymal Stem Cells on Pain Behaviour in an Animal Model of Osteoarthritis Stem Cells Int, 2017-12-24;2017(0):2905104. 2017-12-24 [PMID: 29434641] (Rat)
• R Venkatesan, YU Park, E Ji, EJ Yeo, SY Kim Malathion increases apoptotic cell death by inducing lysosomal membrane permeabilization in N2a neuroblastoma cells: a model for neurodegeneration in Alzheimer's disease Cell Death Discov, 2017-04-24;3(0):17007. 2017-04-24 [PMID: 28487766] (Rat)
• WS Suh, KJ Park, DH Kim, L Subedi, SY Kim, SU Choi, KR Lee A biphenyl derivative from the twigs of Chaenomeles speciosa Bioorg. Chem., 2017-04-11;72(0):156-160. 2017-04-11 [PMID: 28460357] (Rat)
• LA Mesentier-, S De Nicol•, P Rosso, LA De Vitis, V Castoldi, L Leocani, R Mendez-Ote, MF Santiago, P Tirassa, P Rama, A Lambiase Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells Int J Mol Sci, 2017-01-05;18(1):. 2017-01-05 [PMID: 28067793] (Rat)
• Zeng , Wen, Rong , Mengyao, Hu , Xueyu, Xiao , Wei, Qi , Fengyu, Huang , Jinghui, Luo , Zhuojing Incorporation of chitosan microspheres into collagen-chitosan scaffolds for the controlled release of nerve growth factor. PLoS ONE, 2014-07-01;9(7):e101300. 2014-07-01 [PMID: 24983464] (Rat)
• Mousa S, Shaqura M, Khalefa B, Zollner C, Schaad L, Schneider J, Shippenberg T, Richter J, Hellweg R, Shakibaei M, Schafer M Rab7 silencing prevents mu-opioid receptor lysosomal targeting and rescues opioid responsiveness to strengthen diabetic neuropathic pain therapy. Diabetes, 2012-12-10;62(4):1308-19. 2012-12-10 [PMID: 23230081] (Rat)
• Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA. J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87. 2010-02-02 [PMID: 20138055] (Rat)
• Moon E, Her Y, Lee JB, Park JH, Lee EH, Kim SH, Oh MS, Jang CG, Kim SY The multi-herbal medicine Gongjin-dan enhances memory and learning tasks via NGF regulation. Neurosci. Lett., 2009-09-27;466(3):114-9. 2009-09-27 [PMID: 19788912] (Rat)
• Colafrancesco V, Cirulli F, Rossi S, Berry A, Aloe L Anti-NGF-antibody administration as collyrium reduces the presence of NGF and enhances the expression of VEGF in the retina, lacrimal gland and hippocampus. Neurosci. Lett., 2009-08-03;463(3):203-6. 2009-08-03 [PMID: 19651190] (Rat)
• Aloe L, Manni L Low-frequency electro-acupuncture reduces the nociceptive response and the pain mediator enhancement induced by nerve growth factor. Neurosci. Lett., 2008-11-07;449(3):173-7. 2008-11-07 [PMID: 19013501] (Rat)
• Kim SY, Choi JS, Joo CK Effects of nicergoline on corneal epithelial wound healing in rat eyes. Invest. Ophthalmol. Vis. Sci., 2008-10-03;50(2):621-5. 2008-10-03 [PMID: 18836171] (Rat)
• O'Brien LM, Fitzpatrick E, Baird AW, Campion DP Eosinophil-nerve interactions and neuronal plasticity in rat gut associated lymphoid tissue (GALT) in response to enteric parasitism. J. Neuroimmunol., 2008-05-20;197(1):1-9. 2008-05-20 [PMID: 18495257] (Rat)
• Gilmore JH, Jarskog LF, Vadlamudi S Maternal poly I:C exposure during pregnancy regulates TNF alpha, BDNF, and NGF expression in neonatal brain and the maternal-fetal unit of the rat. J. Neuroimmunol., 2004-11-24;159(1):106-12. 2004-11-24 [PMID: 15652408] (Rat)
• Yu G, Xu L, Hadman M, Hess DC, Borlongan CV Intracerebral transplantation of carotid body in rats with transient middle cerebral artery occlusion. Brain Res., 2004-07-23;1015(1):50-6. 2004-07-23 [PMID: 15223366] (Rat)
• Mahmood A, Lu D, Chopp M Intravenous administration of marrow stromal cells (MSCs) increases the expression of growth factors in rat brain after traumatic brain injury. J. Neurotrauma, 2004-01-01;21(1):33-9. 2004-01-01 [PMID: 14987463] (Rat)

FAQs for beta-NGF (DY556). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Ngf