RASGRP2 Antibody - BSA Free

Western Blot: RASGRP2 Antibody [NBP1-33315] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: RASGRP2 Antibody [NBP1-33315] - Human hepatoma. RASGRP2 antibody dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: RASGRP2 Antibody [NBP1-33315] - Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunoprecipitation: RASGRP2 Antibody [NBP1-33315] - Immunoprecipitation of RASGRP2 protein from Raji whole cell extracts using 5 ug of RASGRP2 antibody. Western blot analysis was performed using RASGRP2 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: RASGRP2 Antibody [NBP1-33315] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RASGRP2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Po, Bv, Rb, RM
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RASGRP2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RASGRP2. The exact sequence is proprietary.
• Localization: Cytoplasm, cytosol, Cell membrane, Peripheral membrane protein, Cell junction, synapse, synaptosome (Potential), Cell projection, ruffle membrane, Peripheral membrane protein (Potential)
• Predicted Species: Rat (96%), Porcine (97%), Bovine (95%), Rabbit (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RASGRP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RASGRP2 Antibody - BSA Free

• Calcium and DAG-regulated guanine nucleotide exchange factor I
• CALDAG-GEFI
• CDC25Lcalcium and diacylglycerol-regulated guanine nucleotide exchange factor I
• Cdc25-like protein
• F25B3.3 kinase-like protein
• guanine exchange factor MCG7
• hCDC25L
• MCG7
• RAS guanyl nucleotide-releasing protein 2
• RAS guanyl releasing protein 2 (calcium and DAG-regulated)
• RAS guanyl-releasing protein 2

Background

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RASGRP2