RASGRP2 Antibody

Images

 
Western Blot: RASGRP2 Antibody [NBP1-33315] - Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: RASGRP2 Antibody [NBP1-33315] - Paraffin-embedded hepatoma. RASGRP2 antibody dilution: 1:200.
Western Blot: RASGRP2 Antibody [NBP1-33315] - Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunoprecipitation: RASGRP2 Antibody [NBP1-33315] - Immunoprecipitation of RASGRP2 protein from Raji whole cell extracts using 5 ug of RASGRP2 antibody. Western blot analysis was performed using RASGRP2 antibody. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Rb, RMSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RASGRP2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RASGRP2. The exact sequence is proprietary.
Localization
Cytoplasm, cytosol, Cell membrane, Peripheral membrane protein, Cell junction, synapse, synaptosome (Potential), Cell projection, ruffle membrane, Peripheral membrane protein (Potential)
Predicted Species
Rat (96%), Porcine (97%), Bovine (95%), Rabbit (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RASGRP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RASGRP2 Antibody

  • Calcium and DAG-regulated guanine nucleotide exchange factor I
  • CALDAG-GEFI
  • CDC25Lcalcium and diacylglycerol-regulated guanine nucleotide exchange factor I
  • Cdc25-like protein
  • F25B3.3 kinase-like protein
  • guanine exchange factor MCG7
  • hCDC25L
  • MCG7
  • RAS guanyl nucleotide-releasing protein 2
  • RAS guanyl releasing protein 2 (calcium and DAG-regulated)
  • RAS guanyl-releasing protein 2

Background

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RASGRP2