Rasgrp1 Antibody

Western Blot: Rasgrp1 Antibody [NBP2-94157] - Analysis of extracts of THP-1 cells, using Rasgrp1 antibody (NBP2-94157) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/Immunofluorescence: Rasgrp1 Antibody [NBP2-94157] - Immunofluorescence analysis of NIH/3T3 cells using Rasgrp1 Rabbit pAb (NBP2-94157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: Rasgrp1 Antibody [NBP2-94157] - Immunofluorescence analysis of A-549 cells using Rasgrp1 Rabbit pAb (NBP2-94157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunocytochemistry/Immunofluorescence: Rasgrp1 Antibody [NBP2-94157] - Immunofluorescence analysis of C6 cells using Rasgrp1 Rabbit pAb (NBP2-94157) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rasgrp1 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 598-797 of human RASGRP1 (NP_005730.2). PVAPTENNTSVGPVSNLCSLGAKDLLHAPEEGPFTFPNGEAVEHGEESKDRTIMLMGVSSQKISLRLKRAVAHKATQTESQPWIGSEGPSGPFVLSSPRKTAQDTLYVLPSPTSPCPSPVLVRKRAFVKWENKDSLIKSKEELRHLRLPTYQELEQEINTLKADNDALKIQLKYAQKKIESLQLEKSNHVLAQMEQGDCS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RASGRP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Rasgrp1 Antibody

• RAS guanyl-releasing protein 1
• CALDAG-GEFI
• CALDAG-GEFII
• hRasGRP1
• RAS guanyl releasing protein 1 (calcium and DAG-regulated)
• RASGRP

Background

The Rasgrp1 gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RASGRP1