RanGAP1 Antibody

Western Blot: RanGAP1 Antibody [H00005905-B01P] - Analysis of RANGAP1 expression in Jurkat.

Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody [H00005905-B01P] - Analysis of purified antibody to RANGAP1 on HeLa cell. (antibody concentration 10 ug/ml)

Immunohistochemistry-Paraffin: RanGAP1 Antibody [H00005905-B01P] - Analysis of purified antibody to RANGAP1 on formalin-fixed paraffin-embedded human placenta. (antibody concentration 3 ug/ml)

Western Blot: RanGAP1 Antibody [H00005905-B01P] - Analysis of RANGAP1 expression in transfected 293T cell line by RANGAP1 polyclonal antibody. Lane 1: RANGAP1 transfected lysate(64.57 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

RanGAP1 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: RANGAP1 (NP_002874.1, 1 a.a. - 587 a.a.) full-length human protein. MASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKDVIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRTEIPPALISLGEGLITAGAQLVELDLSDNAFGPDGVQGFEALLKSSACFTLQELKLNNCGMGIGGGKILAAALTECHRKSSAQGKPLALKVFVAGRNRLENDGATALAEAFRVIGTLEEVHMPQNGINHPGITALAQAFAVNPLLRVINLNDNTFTEKGAVAMAETLKTLRQVEVINFGDCLVRSKGAVAIADAIRGGLPKLKELNLSFCEIKRDAALAVAEAMADKAELEKLDLNGNTLGEEGCEQLQEVLEGFNMAKVLASLSDDEDEEEEEEGEEEEEEAEEEEEEDEEEEEEEEEEEEEEPQQRGQGEKSATPSRKILDPNTGEPAPVLSSPPPADVSTFLAFPSPEKLLRLGPKSSVLIAQQTDTSDPEKVVSAFLKVSSVFKDEATVRMAVQDAVDALMQKAFNSSSFNSNTFLTRLLVHMGLLKSEDKVKAIANLYGPLMALNHMVQQDYFPKALAPLLLAFVTKPNSALESCSFARHSLLQTLYKV
• Specificity: Reacts with Ran GTPase activating protein 1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: RANGAP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate in WB, IF, IHC-P and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for RanGAP1 Antibody

• Fug1
• KIAA1835Fug1
• MGC20266
• Ran GTPase activating protein 1
• ran GTPase-activating protein 1
• RanGAP1
• SD
• segregation distorter homolog (Drosophila)
• segregation distorter homolog
• segregation distortion

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RANGAP1
• Entrez:
  • 5905(Human)
• Uniprot:
  • NP_002874.1(Human)