RanGAP1 Antibody (JG37-41)

Western Blot: RanGAP1 Antibody (JG37-41) [NBP2-76865] - Western blot analysis of RanGAP1 on different cell lysates using anti-RanGAP1 antibody at 1/1,000 dilution.Positive control:Lane 1: MCF-7Lane 2: SiHa Lane 3: 293

Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (JG37-41) [NBP2-76865] - ICC staining RanGAP1 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: RanGAP1 Antibody (JG37-41) [NBP2-76865] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.

Flow Cytometry: RanGAP1 Antibody (JG37-41) [NBP2-76865] - Flow cytometric analysis of A431 cells with RanGAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: RanGAP1 Antibody (JG37-41) [NBP2-76865] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: RanGAP1 Antibody (JG37-41) [NBP2-76865] - Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: RanGAP1 Antibody (JG37-41) [NBP2-76865] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-RanGAP1 antibody. Counter stained with hematoxylin.

Product Discontinued

RanGAP1 Antibody (JG37-41) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human RanGAP1 aa 1-50 / 587. (SwissProt: P46060 Human)
• Localization: Cytoplasm. Cytoskeleton. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RANGAP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 64 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RanGAP1 Antibody (JG37-41)

• Fug1
• KIAA1835Fug1
• MGC20266
• Ran GTPase activating protein 1
• ran GTPase-activating protein 1
• RanGAP1
• SD
• segregation distorter homolog (Drosophila)
• segregation distorter homolog
• segregation distortion

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RANGAP1