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RanBP1 Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-79814AF350
Alexa Fluor 405
NB100-79814AF405
Alexa Fluor 488
NB100-79814AF488
Alexa Fluor 532
NB100-79814AF532
Alexa Fluor 594
NB100-79814AF594
Alexa Fluor 647
NB100-79814AF647
Alexa Fluor 700
NB100-79814AF700
Alexa Fluor 750
NB100-79814AF750
Biotin
NB100-79814B
DyLight 350
NB100-79814UV
DyLight 405
NB100-79814V
DyLight 488
NB100-79814G
DyLight 594
NB100-79814DL594
DyLight 650
NB100-79814C
DyLight 680
NB100-79814FR
DyLight 755
NB100-79814IR
FITC
NB100-79814F
HRP
NB100-79814H
Janelia Fluor 549
NB100-79814JF549
Janelia Fluor 646
NB100-79814JF646
Unconjugated
NB100-79814
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RanBP1 Antibody [DyLight 550] Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RANBP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28821644).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RanBP1 Antibody [DyLight 550]

  • HTF9A
  • MGC88701
  • RAN binding protein 1
  • Ran-binding protein 1
  • RanBP1
  • ran-specific GTPase-activating protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Pm
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Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85638] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85638] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
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Western Blot: RanBP2 Antibody [NB120-2938] - Effect of hypoxia on the interaction between HIF1alpha and BICD1. Co-immunoprecipitation of HIF1alpha, Dynein IC, Importin alpha3, RanBP2 with IgG and BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41418-018-0241-1) licensed under a CC-BY licence.
Immunohistochemistry: RanBP2 Antibody [NB120-2938] - Figure 1 illustrates immunohistochemical staining of RanBP2 in bovine retina using NB120-2938. Figure 2 illustrates Western blot detection of RanBP2 from human retinal extracts using NB120-2938.
Western Blot: RanBP2 Antibody [NB120-2938] - Analysis of human retinal extracts.
(2 Publications)
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CRM1 Antibody
NB100-79802
Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] - Association between Nup98-HoxA9 and CRM1 is critical for the Hox Gene activation mediated by Nup98-HoxA9. (B) The effect of LMB treatment on the cellular localization of Nup98-HoxA9. Nup98-HoxA9 ES cells were cultured either in the presence or absence of 5 nM LMB for 2 hr, fixed and stained with antibodies against FLAG (M2) and CRM1. Merged images of FLAG (green) and CRM1 (red) are shown. Nuclei were stained with DAPI. Bar, 10um. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/09540) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
(9 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
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(OTI1B4)
NBP2-02623
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Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.
Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human prostate tissue using anti-RanGAP1 mouse monoclonal antibody.
Species: Hu, Rt, Ca
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SOS2 Antibody
[Unconjugated]
AF6260
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
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(SOS-01)
NB110-68800
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NBP2-95233
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(196908)
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Niemann-Pick C1
Antibody
NB400-148
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Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB
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[Unconjugated]
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Immunoprecipitation: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Detection of human ARID4A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID4A antibody NBP1-26616 used for IP at 3 ug per reaction. ARID4A was also immunoprecipitated by another rabbit anti-ARID4A antibody. For blotting immunoprecipitated ARID4A, NBP1-26616 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Western Blot: EXOSC3 Antibody [NBP1-91874] - Analysis in control (vector only transfected HEK293T lysate) and EXOSC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: EXOSC3 Antibody [NBP1-91874] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
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RanBP1 Antibody
[DyLight 550]
NB100-79814R
RanBP1 Antibody [DyLight 550]
Species: Hu, Mu
Applications: WB, IHC, IHC-P

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-79814AF350
Alexa Fluor 405 NB100-79814AF405
Alexa Fluor 488 NB100-79814AF488
Alexa Fluor 532 NB100-79814AF532
Alexa Fluor 594 NB100-79814AF594
Alexa Fluor 647 NB100-79814AF647
Alexa Fluor 700 NB100-79814AF700
Alexa Fluor 750 NB100-79814AF750
Biotin NB100-79814B
DyLight 350 NB100-79814UV
DyLight 405 NB100-79814V
DyLight 488 NB100-79814G
DyLight 550 NB100-79814R
DyLight 594 NB100-79814DL594
DyLight 650 NB100-79814C
DyLight 680 NB100-79814FR
DyLight 755 NB100-79814IR
FITC NB100-79814F
HRP NB100-79814H
Janelia Fluor 549 NB100-79814JF549
Janelia Fluor 646 NB100-79814JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol RANBP1
Entrez
Uniprot