RAIDD/CRADD Antibody - BSA Free

Western Blot: RAIDD/CRADD Antibody [NBP1-77061] - Whole cell lysates from HeLa (H) or K562 (K) cells with RAIDD antibody at 1:500 dilution.

Immunocytochemistry/ Immunofluorescence: RAIDD/CRADD Antibody - BSA Free [NBP1-77061] - Immunofluorescence of RAIDD/CRADD in Hela cells with RAIDD/CRADD antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: RAIDD/CRADD Antibody - BSA Free [NBP1-77061] - Immunofluorescence of RAIDD/CRADD in Hela cells with RAIDD/CRADD antibody at 20 ug/mL.

Immunocytochemistry/Immunofluorescence: RAIDD/CRADD Antibody [NBP1-77061] - HeLa cells with RAIDD antibody at 5 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RAIDD/CRADD Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 19 amino acid peptide near the center of human RAIDD. The immunogen is located within amino acids 90 - 140 of RAIDD. Amino Acid Squence: AGDRLTGIPSHILNSSPSD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRADD
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5-20 ug/mL
  • Western Blot 0.5-1 ug/mL
• Application Notes: A 22 kDa band should be detected in Western Blot.
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  RAIDD Peptide (NBP1-77061PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for RAIDD/CRADD Antibody - BSA Free

• CASP2 and RIPK1 domain containing adaptor with death domain
• Caspase and RIP adapter with death domain
• caspase and RIP adaptor with death domain
• CRADD
• death domain containing protein CRADD
• death domain-containing protein CRADD
• MGC9163
• RAIDD
• RAIDDdeath adaptor molecule RAIDD
• RIP-associated ICH1/CED3-homologous protein with death domain
• RIP-associated protein with a death domain

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1,RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1,3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRADD
• Uniprot:
  • AAB42217(Human)