Rad9 Antibody

Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Rad9 Antibody Summary

• Immunogen: Peptide with sequence C-QGPSPVLAEDSEGE corresponding to C-Terminus according to NP_004575.1.
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RAD9A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/mL
• Control:

  A-431 Whole Cell Lysate
  Rad9 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rad9 Antibody

• cell cycle checkpoint control protein RAD9A
• DNA repair exonuclease rad9 homolog A
• EC 3.1.11.2
• hRAD9
• RAD9 (S. pombe) homolog
• RAD9 homolog A (S. pombe)
• RAD9

Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad9 Antibody (NB100-57077)(1)

Publication using NB100-57077

• Brandt PD, Helt CE, Keng PC, Bambara RA. The Rad9 protein enhances survival and promotes DNA repair following exposure to ionizing radiation. Biochem Biophys Res Commun 2006-08-18 [PMID: 16814252]

Bioinformatics

• Gene Symbol: RAD9A
• Entrez:
  • 5883(Human)
  • 19367(Mouse)
• Uniprot:
  • NP_004575.1(Human)