Rad9 Antibody (OTI7B11)

Western Blot: Rad9 Antibody (7B11) [NBP1-47946] Analysis of extracts (35ug) from 9 different cell lines by using anti-anti-Rad9monoclonal antibody.

Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded colon tissue using anti-Rad9 mouse monoclonal antibody.

Western Blot: Rad9 Antibody (7B11) [NBP1-47946] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad9.

Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-Rad9 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-Rad9 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-Rad9 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Rad9 Antibody (7B11) [NBP1-47946] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-Rad9 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI7B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

Rad9 Antibody (OTI7B11) Summary

• Description: Novus Biologicals Mouse Rad9 Antibody (OTI7B11) (NBP1-47946) is a monoclonal antibody validated for use in IHC and WB. Anti-Rad9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length protein expressed in 293T cell transfected with human RAD9A expression vector
• Specificity: This antibody is specific for Homo sapiens RAD9 homolog A (S. pombe) (RAD9A).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD9A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 42.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Rad9 Antibody (OTI7B11)

• cell cycle checkpoint control protein RAD9A
• DNA repair exonuclease rad9 homolog A
• EC 3.1.11.2
• hRAD9
• RAD9 (S. pombe) homolog
• RAD9 homolog A (S. pombe)
• RAD9

Background

This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rad9 Antibody (NBP1-47946)(1)

Publication using NBP1-47946

• Yun H, Shi R, Yang Q et al. Over expression of hRad9 protein correlates with reduced chemosensitivity in breast cancer with administration of neoadjuvant chemotherapy Sci Rep. 2014-12-17 [PMID: 25520248] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: RAD9A
• Entrez:
  • 5883(Human)