Rab4 Antibody

Immunocytochemistry/Immunofluorescence: Rab4 Antibody [NB100-74519] - in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAB4 polyclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: Rab4 Antibody [NB100-74519] - Immunohistochemistry was performed on normal deparaffinized human Stomach tissue.

Immunocytochemistry/Immunofluorescence: Rab4 Antibody [NB100-74519] - in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAB4 polyclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: Rab4 Antibody [NB100-74519] - in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAB4 polyclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: Rab4 Antibody [NB100-74519] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.

Product Discontinued

Rab4 Antibody Summary

• Immunogen: Purified, recombinant human Rab4 protein.
• Localization: Peripheral membrane protein, generally associated with membranes. Cytoplasmic when phosphorylated by CDC2.
• Marker: Early and Recycling Endosomes Marker
• Specificity: Rab4
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAB4A
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:1000
• Application Notes: Useful in WB, IF. WB: Detects an approx. 24 kDa protein representing Rab4 from transfected HeLa cells.
• Control:

  Rab4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Rab4 Antibody

• Oncogene RAB4
• RAB4, member RAS oncogene family
• RAB4A, member RAS oncogene family
• RAB4HRES-1/RAB4
• ras-related protein Rab-4A

Background

Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAB4A
• Entrez:
  • 5867(Human)