Rab25 Antibody (467657) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 467657
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Rab25 Antibody (467657) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human RAB25
  Met1-Ala195
  Accession # P57735
• Specificity: Detects human RAB25 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human RAB5A, 10, 11A, 27A, or 27B is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rab25 Antibody (467657) [Alexa Fluor® 647]

• CATX8
• CATX-8RAB11C
• RAB11C
• Rab25
• RAB25, member RAS oncogene family
• ras-related protein Rab-25

Background

Ras-related protein RAB25, also known as CATX-8, is a 23 kDa (unglycosylated) member of the small GTPase superfamily and RAB family of proteins. Human RAB25 is 213 amino acids (aa) in length. The mature chain runs from aa 1-210 with residues 211-213 constituting a propeptide. Residues 10-174 are noted to be RAB11-like. RAB11a, RAB11b, and RAB25 are closely related RAB proteins that are differentially expressed. Human RAB25 shares 96% aa sequence identity with mouse RAB25. Functionally, RAB25 is involved in the regulation of cell survival. Researchers have shown that RAB25 promotes invasive migration of cells and increases the rates and aggressiveness of breast and ovarian cancers. It is expressed in breast and ovarian tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P57735(Human)