Myosin VB Antibody - BSA Free

Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.

Staining of human endometrium, placenta, skeletal muscle and small intestine .

Staining of human placenta shows moderate to strong cytoplasmic and membranous positivity in trophoblastic cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human small intestine shows membranous and cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Myosin VB Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: NLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLK
• Predicted Species: Mouse (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYO5B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Myosin VB Protein (NBP1-87746PEP)

Reactivity Notes

Rat (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Myosin VB Antibody - BSA Free

• KIAA1119
• MYO5B variant protein
• myosin VB
• myosin-Vb

Background

Myosin and Kinesin motor domain. These ATPases belong to the P-loop NTPase family and provide the driving force in myosin and kinesin mediated processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin VB Antibody (NBP1-87746)(18)

Publications using NBP1-87746

• Silverman JB, Krystofiak EE, Caplan LR et Al. Organization of a cytoskeletal superstructure in the apical domain of intestinal tuft cells J Cell Biol 2024-10-01 [PMID: 39352498]
• Gabriele Zaffagnini, Shiya Cheng, Marion C Salzer, Barbara Pernaute, Juan Manuel Duran, Manuel Irimia, Melina Schuh, Elvan Böke Mouse oocytes sequester aggregated proteins in degradative super-organelles. Cell 2024-03-04 [PMID: 38382525]
• Gromova KV, Thies E, Janiesch PC et al. The kinesin Kif21b binds myosin Va and mediates changes in actin dynamics underlying homeostatic synaptic downscaling Cell reports 2023-07-25 [PMID: 37418322] (WB, Mouse)
• Zhao G, Liu S, Arun S et al. A tubule-sheet continuum model for the mechanism of nuclear envelope assembly Developmental cell 2023-04-24 [PMID: 37098350] (WB, Human)
• YW Jun, S Lee, BK Ban, JA Lee, FB Gao Non-muscle MYH10/myosin IIB recruits ESCRT-III to participate in autophagosome closure to maintain neuronal homeostasis Autophagy, 2023-02-27;0(0):1-17. 2023-02-27 [PMID: 36849436]
• Engevik KA, Engevik MA, Engevik AC Bioinformatics reveal elevated levels of Myosin Vb in uterine corpus endometrial carcinoma patients which correlates to increased cell metabolism and poor prognosis PloS one 2023-01-20 [PMID: 36662766] (IHC-P, Human)
• Ahsan MK, Dos Reis DC, Barbieri A et al. Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID) Journal of clinical medicine 2022-07-19 [PMID: 35887942] (IHC-P, Mouse)
• Sakai T, Choo Y, Sato O et al. Myo5b Transports Fibronectin-Containing Vesicles and Facilitates FN1 Secretion from Human Pleural Mesothelial Cells International Journal of Molecular Sciences [PMID: 35563212]
• Li Q, Zhou Z, Sun Y et al. A Functional Relationship Between UNC45A and MYO5B Connects Two Rare Diseases With Shared Enteropathy Cellular and molecular gastroenterology and hepatology 2022-04-11 [PMID: 35421597] (ICC/IF)
• Leijnse N, Barooji YF, Arastoo MR et al. Filopodia rotate and coil by actively generating twist in their actin shaft Nature Communications 2022-03-28 [PMID: 35347113]
• Engevik AC, Coutts AW, Kaji I et al. Editing Myosin VB Gene to Create Porcine Model of Microvillus Inclusion Disease, With Microvillus-Lined Inclusions and Alterations in Sodium Transporters Gastroenterology 2020-02-26 [PMID: 32112796]
• Royo M, Gutierrez Y et al. A retention-release mechanism based on RAB11FIP2 for AMPA receptor synaptic delivery during long-term potentiation. J Cell Sci 2019-12-16 [PMID: 31757887] (ICC/IF, Rat)
• Forteza R, Ahsan MK, CartOn-GarcIa F et al. Glucocorticoids and Myosin5b loss-of-function induce heightened PKA signaling in addition to membrane traffic defects Mol. Biol. Cell 2019-10-30 [PMID: 31664880] (KO, Human, Mouse)
• Xu, W;Gulvady, AC;Goreczny, GJ;Olson, EC;Turner, CE; Paxillin-dependent regulation of apical-basal polarity in mammary gland morphogenesis Development 2019-04-09 [PMID: 30967426] (WB, Mouse)
• Kravtsov DV, Ahsan MK, Kumari V et al. Identification of Intestinal Ion Transport Defects in Microvillus Inclusion Disease. Am. J. Physiol. Gastrointest. Liver Physiol. 2016-05-26 [PMID: 27229121] (WB, ICC/IF, Human)
• Schneeberger, K, Vogel, G F Et al. An inducible mouse model for microvillus inclusion disease reveals a role for myosin Vb in apical and basolateral trafficking. Proc Natl Acad Sci U S A 2015-10-06 [PMID: 26392529] (WB, Mouse)
• Carton-Garcia F, Overeem AW, Nieto R et al. Myo5b knockout mice as a model of microvillus inclusion disease. Sci Rep 2015-07-23 [PMID: 26201991] (IF/IHC, Mouse)
• Kravtsov D, Mashukova A, Forteza R et al. Myosin 5b loss of function leads to defects in polarized signalling: Implication for Microvillus Inclusion Disease pathogenesis and treatment. Am. J Physiol. Gastrointest. Liver Physiol. 2014-09-25 [PMID: 25258405]

Bioinformatics

• Gene Symbol: MYO5B