Myosin VB Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues. Corresponding MYO5B RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium, placenta, skeletal muscle and small intestine using Anti-MYO5B antibody NBP1-87746 (A) shows ...read more
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
Simple Western: Myosin VB Antibody [NBP1-87746] - Analysis in RT4 at 0.2 mg/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Myosin VB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLK
Specificity
Specificity of human Myosin VB antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYO5B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04 - 0.4 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockout Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. WB, ICC/IF reported in the scientific literature (PMID: 27229121). Knockout validation (PMID: 31664880).
Control Peptide
Myosin VB Protein (NBP1-87746PEP)
Publications
Read Publications using
NBP1-87746 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • KO
    1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26201991). Rat (89%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Myosin VB Antibody

  • KIAA1119
  • MYO5B variant protein
  • myosin VB
  • myosin-Vb

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin VB Antibody (NBP1-87746)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, KO, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
KO
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-87746 Applications Species
Engevik AC, Coutts AW, Kaji I et al. Editing Myosin VB Gene to Create Porcine Model of Microvillus Inclusion Disease, With Microvillus-Lined Inclusions and Alterations in Sodium Transporters Gastroenterology Feb 26 2020 [PMID: 32112796]
Royo M, Gutierrez Y et al. A retention-release mechanism based on RAB11FIP2 for AMPA receptor synaptic delivery during long-term potentiation. J Cell Sci Dec 16 2019 [PMID: 31757887] (ICC/IF, Rat) ICC/IF Rat
Forteza R, Ahsan MK, CartOn-GarcIa F et al. Glucocorticoids and Myosin5b loss-of-function induce heightened PKA signaling in addition to membrane traffic defects Mol. Biol. Cell Oct 30 2019 [PMID: 31664880] (KO, Mouse, Human) KO Mouse, Human
Xu, W;Gulvady, AC;Goreczny, GJ;Olson, EC;Turner, CE; Paxillin-dependent regulation of apical-basal polarity in mammary gland morphogenesis Development Apr 9 2019 [PMID: 30967426] (WB, Mouse) WB Mouse
Kravtsov DV, Ahsan MK, Kumari V et al. Identification of Intestinal Ion Transport Defects in Microvillus Inclusion Disease. Am. J. Physiol. Gastrointest. Liver Physiol. May 26 2016 [PMID: 27229121] (WB, ICC/IF, Human) WB, ICC/IF Human
Carton-Garcia F, Overeem AW, Nieto R et al. Myo5b knockout mice as a model of microvillus inclusion disease. Sci Rep 2015 Jul 23 [PMID: 26201991] (IHC, Mouse) IHC Mouse
Kravtsov D, Mashukova A, Forteza R et al. Myosin 5b loss of function leads to defects in polarized signalling: Implication for Microvillus Inclusion Disease pathogenesis and treatment. Am. J Physiol. Gastrointest. Liver Physiol. 2014 Sep 25 [PMID: 25258405]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MYO5B