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Myosin VB Antibody

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Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human colon, kidney, lymph node and testis using Anti-MYO5B antibody NBP1-87746 (A) shows similar protein distribution across tissues to independent antibody NBP1-87747 (B).
Independent Antibodies: Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human colon, kidney, lymph node and testis using Anti-MYO5B antibody NBP1-87746 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human cervix, uterine shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues. Corresponding MYO5B RNA-seq data are presented for the same ...read more
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human lymph node using Anti-MYO5B antibody.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human kidney using Anti-MYO5B antibody.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human colon using Anti-MYO5B antibody.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human testis using Anti-MYO5B antibody.
Simple Western: Myosin VB Antibody [NBP1-87746] - Analysis in RT4 at 0.2 mg/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

Order Details

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Myosin VB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: NLMKKELEEERSRYQNLVKEYSQLEQRYDNLRDEMTIIKQTPGHRRNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGLEKAAMDMTVFLK
Specificity
Specificity of human Myosin VB antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MYO5B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockout Validated
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. WB, ICC/IF reported in the scientific literature (PMID: 27229121). Knockout validation (PMID: 31664880).
Control Peptide
Publications
Read Publications using
NBP1-87746 in the following applications:

  • ICC/IF
    1 publication
  • IHC
    1 publication
  • KO
    1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26201991). Rat (89%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Myosin VB Antibody

  • KIAA1119
  • MYO5B variant protein
  • myosin VB
  • myosin-Vb

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Myosin VB Antibody
NBP1-87746
Myosin VB Antibody
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KO

Publications for Myosin VB Antibody (NBP1-87746)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, KO, WB.


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Bioinformatics

Gene Symbol MYO5B
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