Rab25 Antibody (467657) [Alexa Fluor® 700]

• Applications: WB
• Clone: 467657
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

Rab25 Antibody (467657) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human RAB25
  Met1-Ala195
  Accession # P57735
• Specificity: Detects human RAB25 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human RAB5A, 10, 11A, 27A, or 27B is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Ras-related protein RAB25, also known as CATX-8, is a 23 kDa (unglycosylated) member of the small GTPase superfamily and RAB family of proteins. Human RAB25 is 213 amino acids (aa) in length. The mature chain runs from aa 1-210 with residues 211-213 constituting a propeptide. Residues 10-174 are noted to be RAB11-like. RAB11a, RAB11b, and RAB25 are closely related RAB proteins that are differentially expressed. Human RAB25 shares 96% aa sequence identity with mouse RAB25. Functionally, RAB25 is involved in the regulation of cell survival. Researchers have shown that RAB25 promotes invasive migration of cells and increases the rates and aggressiveness of breast and ovarian cancers. It is expressed in breast and ovarian tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.