Rab13 Antibody (863028) [Unconjugated]

Western blot shows lysates of human lung tissue and human aorta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Rab13 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Rab13 was detected in immersion fixed Caco-2 human colorectal adenocarcinoma cell line using Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western blot shows lysates of HEK-293T parental cell line and Rab13 knockout HEK-293T cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated secondary antibody. A specific band was detected for Rab13 at approximately 23 kDa (as indicated) in the parental HEK-293T cell line, but is not detectable in knockout HEK-293T cell line. Primary antibody dilution used: 2.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Western blot shows lysates of U87 MG parental cell line and Rab13 knockout U87 MG cell line (KD). Nitrocellulose membrane was probed with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated secondary antibody. A specific band was detected for Rab13 at approximately 23 kDa (as indicated) in the parental U87 MG cell line, but is significantly reduced in the U87 MG cell line. Primary antibody dilution used: 2.5 µg/mL. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

U‑87 MG human glioblastoma/astrocytoma parental cell line WT and Rab13 U-87 MG KD cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KD cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KD cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

• Reactivity: Hu
• Applications: WB, ICC/IF, KO
• Clone: 863028
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Rab13 Antibody (863028) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Rab13
  Lys94-Thr191
  Accession # P51153
• Specificity: Detects human Rab13 in direct ELISA and Western Blot.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAB13
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rab13 Antibody (863028) [Unconjugated]

• Cell growth-inhibiting gene 4 protein
• growth-inhibiting gene 4 protein
• Rab13
• RAB13, member RAS oncogene family
• RAS-associated protein RAB13
• ras-related protein Rab-13

Background

Ras-related protein Rab13 is a 23 kDa member of the Rab family of small GTPases.  With more than 40 family members, Rab proteins are regulators of vesicular transport, and their number reflects the complexity of vesicle trafficking.  Rab13 is detected in endothelial cells and a variety of epithelia, including intestine, lung, kidney and liver.  Rab13 may participate in the assembly and activity of tight junctions, as it colocalizes with the marker ZO-1 in polarized epithelial cells.  In addition, insulin promotes GTP loading of Rab13, potentially regulating GLUT4 translocation in skeletal muscle cells.  Human Rab13 is 203 amino acids (aa) in length, with residues 201-203 removed in its mature form.  Over aa 94-191, human Rab13 shares 89 and 90% identity with mouse and rat Rab13, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rab13 Antibody (MAB8305)(1)

Publication using MAB8305

• DR Gulbranson, EM Davis, BA Demmitt, Y Ouyang, Y Ye, H Yu, J Shen RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis Proc. Natl. Acad. Sci. U.S.A., 2017-09-11;0(0):. 2017-09-11 [PMID: 28894007] (Western Blot, Human)

Bioinformatics

• Gene Symbol: RAB13
• Uniprot:
  • P51153()